The invention provides a preparation method of high-strength nylon 6 filaments based on a one-step drafting process. The preparation method is characterized by comprising following steps: step 1, polyol and nylon 6 slices are mixed in proportion, mixed polyol / nylon 6 slices are fed into a twin-screw extruder for melt blending, extruded melt is pelletized by a pelletizer, and polyol modified nylon6 masterbatches are obtained; step 2, the nylon 6 masterbatches obtained in step 1 and the nylon 6 slices are mixed in proportion, the mixture is dried and then subjected to melt extrusion by the twin-screw extruder and enters a spinning manifold, melt is sprayed out through spinneret orifices, then enters a spinning channel and is subjected to air cooling, bundling, oiling, drafting, heat settingand coiling, and the high-strength nylon 6 filaments are prepared. Polyol is added for plasticizing modification of nylon 6, high-strength nylon 6 is prepared with the one-step drafting process, andthe prepared product has excellent thermodynamic properties, higher breaking strength and elongation at break and lower initial modulus.