The embodiment of the invention discloses a moisturizing and oxidation-resisting donkey-hide gelatin essence lotion. The donkey-hide gelatin essence lotion comprises ethylene diamine tetraacetic acid(EDTA) disodium, glycerol, dipropylene glycol, xanthan gum, allantoin, dipotassium glycyrrhizinate, p-hydroxyacetophenone, hexylene glycol, caprylic/capric triglyceride, cetyl ethyl hexanoate, jojobaseed oil, shea butter, cetostearyl alcohol, glyceryl stearate, glyceryl stearate/PEG-100 stearate, stearic acid, tocopherol acetate, polydimethylsiloxane, jojoba esters/acacia decurrens flower wax/sunflower seed wax/polyglycerol-3, polyacrylamide/C13-14 isoparaffin/laureth-7, collagen extract, placental protein and water. The embodiment of the invention further discloses a preparation method for the moisturizing and oxidation-resisting donkey-hide gelatin essence lotion, and the moisturizing and oxidation-resisting donkey-hide gelatin essence lotion prepared by the method has functions of multiple moisturizing, resisting oxidation and improving skin roughness, dullness and fine wrinkles.