The invention discloses high-nutrition soy sauce flavor-type marinated donkey meat and a preparation method thereof. The donkey meat is prepared from the following raw materials: donkey meat, water, Baijiu, monosodium glutamate, salt, white granulated sugar, thick broad-bean sauce, star anise, cassia bark, fructus amomi, alpinia katsumadai, mint, semen plantaginis, radix angelicae, clove, wormwood, flos magnoliae liliflorae, astragalus membranaceus, cinnamon, common aucklandia roots, pericarpium citri reticulatae, liquorice, hawthorn, Chinese wolfberry, jujubes, chilies, lalang grass rhizome,walnut kernel powder, peanut powder, Chinese prickly ash, myrcia, pepper, raw ginger and cumin powder. The method comprises the following steps: cleaning the donkey meat, punching the donkey meat, preparing soup, pre-cooking the donkey meat, and putting the donkey meat into the soup for cooking to obtain the high-nutrition soy sauce flavor-type marinated donkey meat. The soup is high in nutritional value, the nutritional value of the donkey meat is improved, the two sides of the donkey meat can be punched, manual punching of the donkey meat is not needed, the labor intensity is reduced, the production efficiency of the high-nutrition soy sauce flavor-type marinated donkey meat is improved, and the donkey meat can be completely tasty.