The invention relates to the field of animal feeds for livestock and poultry, and especially relates to a feed added with a protein-mulberry leaf additive for reducing cholesterol contents of livestock meat, poultry meat and eggs as well as a preparation method thereof. The feed added with the protein-mulberry leaf additive for reducing the cholesterol contents of the livestock meat, the poultry meat and the eggs comprises the following raw-material ingredients in parts by weight: 35-60 parts of protein-mulberry leaves, 15-20 parts of pine needle powder, 1.2-2.0 parts of dendranthema indicum, 2.0-2.7 parts of wormwood, 1.0-1.5 parts of eucommia ulmoides leaves, 1.0-1.5 parts of lotus leaves, 10-20 parts of rice bran, 10-20 parts of corn powder and 10-30 parts of fermentation broth of live bacteria. The protein-mulberry leaves, the dendranthema indicum, the wormwood, the pine needle powder, the eucommia ulmoides leaves, the lotus leaves and so on are mixed according to a certain ratio in the preparation method, and the mixture is then fermented; thus, the fermented materials are enabled to exert synergistic effects, and the feed is capable of improving qualities of the livestock meat, the poultry meat and the eggs. Moreover, the feed is capable of enhancing antioxidant capabilities of living bodies and slaughtered muscle tissues of the livestock and the poultry, so as to reduce oxidation products and prevent fatty acid formation in the meat; thus, the feed greatly reduces the low-density cholesterol contents in the livestock bodies and the poultry bodies.