122 results about "Phosphoramides" patented technology

The invention relates to the technical field of rubber material preparation, and discloses a flame-retardant environment-friendly rubber material and a preparation method thereof. The preparation method comprises the following steps: carrying out ball milling on phosphamide bis-hexafluorophosphate ionic liquid and a carbon nanotube to obtain an ionic liquid modified carbon nanotube; and mixing with silicone rubber, a vulcanizing agent and the like, and vulcanizing to obtain the flame-retardant environment-friendly rubber material. The phosphamide bis-hexafluorophosphate ionic liquid contains a plurality of hexafluorophosphate ionic groups, and has a large interaction force with the carbon nanotubes, so the surface modification of the carbon nanotubes is effectively realized, the dispersibility of the carbon nanotubes in a silicone rubber matrix is improved, and the tensile property, hardness and other mechanical properties of the silicone rubber are improved. The ionic liquid contains phosphamide and imidazole groups to form a nitrogen-phosphorus flame retardant, and the nitrogen-phosphorus flame retardant and the carbon nanotubes play a synergistic flame-retardant role, so that the flame-retardant property of the rubber is remarkably improved.

The invention provides a non-aqueous electrolyte and a lithium ion battery. The non-aqueous electrolyte comprises a lithium salt, a non-aqueous organic solvent and an additive. The additive comprises a compound A as shown in a structural formula I; wherein R1 and R2 are respectively and independently selected from oxygen, sulfur or NR5, R3 and R4 are respectively and independently selected from NR6R7, R5 is C1-C5 alkyl silicon group, and R6 and R7 are respectively and independently selected from C1-C5 alkyl silicon group, C1-C5 alkyl group, substituted C1-C5 alkyl group, phenyl or substituted phenyl. The compound A comprises a cyclic (oxygen, sulfur and nitrogen) phosphamide structure and a trimethylsilyl structure, the trimethylsilyl structure can remove moisture in the electrolyte, and the cyclic (oxygen, sulfur and nitrogen) phosphamide structure is reduced on a negative electrode interface to form a polar solid electrolyte interface film of lithium salt containing oxygen or sulfur and phosphorus and nitrogen; the interfacial film has good lithium ion conductivity and toughness, the charge transfer resistance of the lithium ion battery is reduced, desolvation of lithium ions is promoted, and the high-rate cycle performance of the lithium ion battery is improved. The structural formula I of the # imgabs0 # is shown in the specification.

The invention belongs to the technical field of battery electrolyte, and particularly relates to flame-retardant cyclic phosphamide eutectic electrolyte as well as a preparation method and application thereof. According to the invention, through reasonable molecular design, the flame-retardant cyclic phosphamide eutectic electrolyte is obtained. Through the design of the annular structure, a film is formed on the positive and negative electrode interface of the electrode in the battery operation process, so that the stability of the battery is improved. Phosphamide groups are introduced into the cyclic structure, hydroxyl radicals generated in the battery operation process are captured through phosphorus-oxygen bonds, and the chain reaction of combustion is blocked. Meanwhile, different designs of amino groups on phosphamide can achieve a self-cleaning effect, hydrofluoric acid and water generated in a battery circulation process are removed, and the battery is protected from electrode structure collapse caused by the hydrofluoric acid and the water in an operation process. The safety performance of the lithium battery in the cycle process is obviously improved, and the development of the lithium battery with high safety and stable operation is effectively promoted. The preparation process is simple, safe to operate, high in purity and suitable for industrialization.

The invention belongs to the technical field of coupling reagents, and particularly discloses application of N-sulfonyl imidazolium salt as a coupling reagent, and the N-sulfonyl imidazolium salt as the coupling reagent is applied to synthesis of amide, dipeptide, polypeptide, carboxylic ester, carboxylic thioester, phosphamide and phosphate. According to the application of the N-sulfonyl imidazolium salt as the coupling reagent, compared with the prior art, the dosage of the coupling reagent is greatly reduced, the generation of byproducts is reduced, and the conversion rate of reactants is improved.

The invention relates to the technical field of biological medicines, and particularly discloses synthesis and application of oligonucleotide with a spacer group embedded between 5-terminal oxygen and thiophosphoric acid. The chemical modification strategy comprises the following steps: preparing a modified nucleotide phosphoramidite monomer in which a spacer group is embedded between O5'and thiophosphoric acid P (V); performing solid-phase synthesis on the modified nucleotide phosphoramidite monomer to construct a target oligonucleotide molecule; a spacer group is embedded between oxygen at the 5 '-terminal of the oligonucleotide and thiophosphoric acid P (V) to form a 5'-terminal thiophosphoric acid structure; as the terminal modified thiophosphoric acid belongs to a non-phosphatase substrate, the terminal modified thiophosphoric acid can resist exonuclease degradation and improve the biological activity of siRNA after being modified.

To provide a method for preparing oligonucleotides that can improve yields and purities of target phosphorodiamidate morpholino oligomers and reduce 4-nitrostyrene adduct impurities.SOLUTION: The process comprises: (a) converting a compound of Formula X-1 into a compound of Formula X-2: where R10 is a residue of an oligonucleotide (e.g., a phosphorodiamidate morpholino oligomer); R11 is an amine protecting group; wherein the compound of Formula X-1 is not bound to a solid support; and (b) optionally removing protecting groups in the compound of Formula X-2 to obtain the olignucleotide.SELECTED DRAWING: None

The invention discloses a method for detecting 5 '(E)-VPA phosphoramidite monomer related substances, and belongs to the technical field of liquid chromatography detection. Related substances comprise 5 '-(E)-VP-2'-OMe-A (Bz), 5 '(Z)-VPA, 5'-(E)-VP-2 '-OMe-A-CE-P and 5' (E)-VPA-OX, and chromatographic detection conditions comprise that the column temperature is 23-27 DEG C, a stationary phase of a chromatographic column is octadecyl bonded silica gel, a mobile phase A is an ammonium acetate solution, a mobile phase B is acetonitrile, gradient elution is performed, and the like. According to the method, four related substances in the 5 '(E)-VPA phosphoramidite monomer are effectively separated through specific liquid chromatography conditions, the system adaptability, specificity, linearity and range, detection limit, quantitation limit and accuracy verification of the method all meet acceptable standards of pharmacopoeia, and detection of multiple related substances can be completed within a short time; and a reliable means is provided for detecting and controlling the quality of the 5 '(E)-VPA phosphoramidite monomer.

An object is to provide an amidite monomer of diaminopurine and / or an amidite monomer of a thiouracil derivative suitable for use in synthesis of an acyclic single-stranded polynucleotide comprising diaminopurine and / or a thiouracil derivative by a phosphoramidite method. This object is achieved by an amidite monomer of diaminopurine and / or an amidite monomer of a thiouracil derivative protected with a specific protecting group removable by a base.

The present invention relates to small molecules having high affinity and specificity to prostrate-specific membrane antigen (PSMA) and methods of using them for therapeutic and diagnostic purposes.

The invention belongs to the technical field of medicinal chemistry, and relates to a novel small nucleic acid drug intermediate and a preparation method thereof, the intermediate is a 2 '-FANA-dU phosphoramidite monomer, the preparation method comprises the following steps: 1, reacting a compound a with a 33% hydrogen bromide-acetic acid solution in dichloromethane, and performing post-treatment to obtain a compound b; 2, reacting uracil and the like to generate a white solid, and reacting the white solid with the compound b to obtain a compound c; 3, reacting the compound c in methanol and ammonia water, crystallizing and drying to obtain a compound d; 4, the compound d reacts with 4, 4 '-dimethoxytriphenylchloromethane, and a compound e is obtained through treatment; 5, the compound e reacts with anhydrous diisopropyl imidazole and a 2-cyanoethyl-N, N, N ', N'-tetraisopropyl phosphoramidite reagent, and the 2 '-FANA-dU phosphoramidite monomer is obtained.The 2'-FANA-dU phosphoramidite monomer prepared through the method can modify siRNA, the biological activity of the siRNA can be reserved or even enhanced, and the biological stability of the siRNA can be remarkably improved.

The invention discloses synthesis of 5-nucleotide phosphorodithioamide and application of the 5-nucleotide phosphorodithioamide in oligonucleotide. The oligonucleotide modified by the phosphorodithioamide comprises nucleotide structural units shown in a formula I or a formula II,..., the formula I,..., the formula II. According to the invention, through specific limitation of the structure of a nucleotide dithiophosphoramide monomer, the prepared oligonucleotide comprises a 5-nucleotide dithiophosphoramide structural unit. Compared with a traditional oligonucleotide molecule, the oligonucleotide molecule modified by nucleotide dithiophosphoramide shows better enzymatic degradation resistance and pharmaceutical stability. In addition, the binding performance between the modified oligonucleotide molecules and environmental ions can be improved through nucleotide dithiophosphamide modification, and a novel chemical modification strategy is provided for research, development and application of oligonucleotide drugs.

The invention discloses synthesis of 5 '-amino-3-nucleotide phosphamide and application of the 5'-amino-3-nucleotide phosphamide in oligonucleotide. The phosphamide modified oligonucleotide comprises a nucleotide structural unit shown as a formula I or a formula II,..., the formula I; ... formula II; through specific limitation of a nucleotide phosphamide monomer structure, the prepared oligonucleotide contains a phosphamide structural unit; compared with a traditional oligonucleotide molecule, the oligonucleotide molecule with the phosphamide modified nucleotide structure has more excellent effects on pharmaceutical parameters such as enzymatic degradation resistance, stability and the like, and in addition, phosphamide modification can improve the binding performance between the modified oligonucleotide molecule and environmental ions; a novel chemical modification strategy is provided for research, development and application of oligonucleotide drugs.

The invention provides a non-aqueous electrolyte and a lithium ion battery thereof. The non-aqueous electrolyte comprises an electrolyte salt, a non-aqueous organic solvent and an additive, wherein the additive comprises a bis (aziridinyl) phosphamide compound as shown in a structural formula I. Wherein R1 is selected from substituted or unsubstituted C1-C6 alkyl or substituted amino, the structural formula of the substituted amino is shown as a structural formula II or a structural formula III, R2, R3, R4, R5, R6, R7, R8, R9, R13, R14, R15 and R16 are respectively and independently selected from hydrogen and substituted or unsubstituted C1-C6 alkyl, and R11 and R12 are respectively and independently selected from substituted or unsubstituted C1-C6 alkyl. The mass ratio of the bis (aziridinyl) phosphamide compound is 0.01 to 1.00 percent. The non-aqueous electrolyte contains a specific flame retardant, can realize excellent flame retardant property only by low content, and has the flame retardant property and better high-temperature cycle and high-temperature storage performance when being used in the lithium ion battery. Structural formula I, structural formula II and structural formula III

The application discloses a five-membered ring containing alkynyl or triazole functional group P The chiral phosphine oxide compound is obtained through Domino Heck-Sonogashira cascade reaction by taking a prochiral tertiary phosphine oxide compound containing a double ethylene group and phenylacetylene as raw materials, transition metal palladium catalyst and chiral TADDOL phosphoramidite ligand under the protection of inert gas P The chiral phosphine oxide compound has a product yield of 96% and an enantioselectivity ee value of 99%, and a diastereoselectivity dr value of greater than 20:1. The application adopts an asymmetric desymmetrization strategy, uses a Heck reaction to start and a Sonogashira reaction to end a cascade reaction, simultaneously constructs P The chiral and quaternary carbon chiral center has mild reaction conditions, simple operation and good substrate universality, provides a compound skeleton with diversity, and is successfully applied to a palladium-catalyzed asymmetric allyl substitution reaction as a chiral ligand.

Provided is an oligonucleotide having excellent stability and capable of inducing ADAR editing activity in a cell. The oligonucleotide comprises a first oligonucleotide and a second oligonucleotide linked to the 5'side thereof, and induces site-specific editing against a target RNA. The first oligonucleotide is composed of a target-corresponding nucleotide residue, an oligonucleotide having 3 to 6 residues complementary to the target RNA on the 5'side thereof, and an oligonucleotide having 10 to 24 residues complementary to the target RNA on the 3 'side thereof. And at least one of a phosphorus-containing linking group connecting the nucleoside residues at the first position and the second position and a phosphorus-containing linking group connecting the nucleoside residues at the fourth position and the fifth position is an alkyl phosphonic acid residue, an alkyl phosphate residue or a substituted phosphamide residue. The second oligonucleotide has a nucleotide residue corresponding to the target RNA deleted at the 3'terminal, or has a nucleotide residue that does not form a complementary pair with the target RNA, the number of residues being 2 to 10, and the nucleotide residues other than the 3 'terminal being complementary to the target RNA.

This invention relates to the field of polyester materials technology, and discloses a flame-retardant biodegradable polyester material and its preparation method. The polyester material of this invention is composed of 65-80 parts by weight of aromatic polyester, 20-35 parts by weight of aliphatic polyester, and 5-25 parts by weight of polylactic acid-based flame retardant. The aromatic polyester is selected from polyethylene terephthalate (PET), and the aliphatic polyester includes biodegradable components such as polylactic acid (PLA). The PLA-based flame retardant of this invention improves the compatibility between aliphatic polyesters such as PLA and aromatic polyesters such as PET, resulting in better mechanical properties. Simultaneously, the main chain contains a large number of phosphoramide groups, forming a nitrogen-phosphorus flame-retardant system, thereby improving the flame-retardant performance of the polyester material.

A process for the hydrocyanation of an organic compound containing at least one olefinic group comprising reacting the organic compound with hydrogen cyanide in the presence of a catalyst complex comprising at least one transition metal and a phosphorus-containing ligand comprising a calixarene backbone and at least two aryl phosphite or aryl phosphoramidite groups chemically bonded to the backbone.

The invention relates to the technical field of compound synthesis, in particular to a method for directly reducing carboxylic acid into aldehyde. The preparation method comprises the following steps: mixing triphenylphosphine, dichloromethane, carboxylic acid and N-chlorosuccinimide, and reacting to obtain a reactant; mixing with pinacol borane and 4-dimethyl pyridine, and continuously reacting to obtain a continuous reactant; and separating and purifying to obtain aldehyde. The synthesis of the substrate carboxylic acid provided by the invention and the rapid deoxidation of the carboxylic acid are carried out to obtain an aldehyde product, especially the following compounds 2aa, 2ab, 2ae, 2bc, 2be, 2bf, 2bg, 2bh, 2bi, 2bj, 2bk and 2bl have catalytic hydrolysis effects on hypophosphamide; the compound 2bl has a particularly excellent effect on hydrolysis of hypophosphamide.

This invention provides an oligonucleotide cleavage reagent (JS2 & S4 reagent) and its preparation method, as well as a method for cleaving oligonucleotides. The oligonucleotide cleavage reagent comprises NaOH, KOH, methanol, and a cleavage buffer. The oligonucleotide cleavage reagent JS2 & S4 and the cleavage method using it provided by this invention can efficiently cleave oligonucleotides, especially those synthesized using solid-phase phosphorous acid cleavage. This reduces chain breakage and side reactions caused by high-temperature cleavage, and also reduces equipment costs. While shortening the cleavage time, it can effectively reduce oligonucleotide quality problems caused by cleavage.

The invention discloses an ultra-dispersed ceramic white switch panel master batch as well as a preparation method and application thereof. Relates to the field of polymer materials. The ultra-dispersed ceramic white switch panel master batch comprises the following components: carrier resin, a modified flame retardant, a dispersing agent and a pigment, molecules of the modified flame retardant contain a triazine ring, benzyl, a phosphamide group and chloroethyl, and the benzyl serves as a connecting unit and is connected with the triazine ring, the phosphamide group and the chloroethyl. According to the ultra-dispersed ceramic white switch panel master batch disclosed by the invention, multi-performance optimization is realized through a synergistic effect of specific components.

The disclosure relates to the production of highly pure N,N-diisopropylphosphoramidite compounds, such as 6-bromohexyl 2-cyanoethyl N,N-diisopropylphosphoramidite, and highly pure starting material used therein, such as 6-bromohexan-1-ol. The resulting N,N- diisopropylphosphoramidite compounds are useful, for example, for the improved preparation of expandable nucleotide triphosphates (XNTPs) for use in sequencing by expansion.

The invention discloses high-strength concrete and a curing method thereof, and belongs to the technical field of concrete. The high-strength concrete comprises the following components: 300-400 kg / m < 3 > of cement, 100-130 kg / m < 3 > of silica fume, 50-80 kg / m < 3 > of fly ash, 700-850 kg / m < 3 > of sand, 850-950 kg / m < 3 > of gravel, 5-9 kg / m < 3 > of a modified polycarboxylate superplasticizer and 140-170 kg / m < 3 > of water. The modified polycarboxylate superplasticizer is formed by copolymerizing a methacrylate functional monomer containing a phosphamide anchoring group, an imidazolium zwitterionic monomer containing a styryl group, an acrylic monomer, a monoethyl fumarate monomer and an allyl polyoxyethylene ether monomer. Through molecular design, a strong anchoring phosphamide group and a mud-resistant zwitterionic structural monomer are introduced into the polycarboxylate superplasticizer, so that the strength of concrete is improved, and the service life of the concrete is prolonged.

The invention relates to the technical field of biological medicine, and particularly discloses synthesis and application of oligonucleotide with threose nucleic acid 3-oxygen at the tail end indirectly connected with thiophosphoric acid. The chemical modification strategy comprises the following steps: O < 3->-oxygen of threose nucleic acid is connected with thiophosphoric acid through a spacer group, and O < 2->-hydroxyl is combined with a nucleotide monomer of phosphoramidite; carrying out solid-phase synthesis on the threose nucleic acid O2 '-phosphoramidite monomer to construct an oligonucleotide molecule; the oxygen of the threose nucleic acid at the terminal of the oligonucleotide is linked to the thiophosphoric acid through a spacer group, and since the type of phosphonic acid does not belong to a substrate of phosphatase, the modified oligonucleotide can resist exonuclease degradation and improve its in vivo biological activity.

Methods are disclosed for making a 4′-phosphate analog phosphoramidite known as MeMOP, which can be used in the synthesis of oligonucleotides such as therapeutic oligonucleotides.

The application provides an imidazole-phosphoramidite bidentate ligand, a synthesis method thereof, a metal catalyst prepared from the imidazole-phosphoramidite bidentate ligand and application of the metal catalyst, and relates to the technical field of catalyst preparation.The imidazole-phosphoramidite bidentate ligand developed by the application has stable chemical properties and is not easy to be oxidized, and solves the problems of poor stability of an imidazole-based bidentate ligand, harsh use and storage conditions in the prior art, and the synthesis method is simple, and the total yield can reach 59%; the imidazole-phosphoramidite bidentate ligand has strong coordination ability, the metal nickel catalyst prepared from the imidazole-phosphoramidite bidentate ligand has high catalytic activity, and in a carbon dioxide carboxylation reaction, the metal nickel catalyst exhibits excellent reaction activity, and is a ligand with very good industrial application prospect.

The invention provides a method for efficiently preparing various substituted chiral phosphoramides, which comprises the following steps: in the presence of an organic solvent, alkali, a chiral ligand and a catalyst, reacting a nucleoside compound or alcohol with racemic amino phosphoryl dichloride or alkoxy phosphoryl dichloride to generate chiral phosphoryl chloride; and reacting the chiral phosphoryl chloride with a nucleophilic reagent to obtain the chiral phosphamide. The synthesis method provided by the invention is simple to operate, high in yield and good in diastereoselectivity or enantioselectivity, and the obtained target compound has the characteristics of good functional group compatibility and wide substrate universality. The obtained product is diversified in structure and good in controllability, and has good application potential in the field of medicine synthesis.

The application provides a nucleoside dimer, a preparation method thereof and application in DNA synthesis. The nucleoside dimer of the application adopts a mixed skeleton of methyl phosphate and beta-cyanoethyl phosphoramidite, a new preparation method of the nucleoside dimer is obtained by optimizing a synthesis method and a purification process, and a foundation for high-quality DNA synthesis is laid. On this basis, the nucleoside dimer with the new skeleton is applied to DNA synthesis, an oligonucleotide fragment synthesis method suitable for the dimer is established, and an oligonucleotide fragment with a length of 100 nt is synthesized. Fidelity experiment shows that the error rate of the DNA fragment synthesized by using the dimer is only 3.75 errors / Kb, and high-fidelity DNA synthesis is realized. The preparation method of the nucleoside dimer with the new skeleton can realize large-scale and high-purity preparation, the nucleoside dimer can play an important role in improving the length and fidelity of oligonucleotide synthesis, and has practical value.

The invention relates to the technical field of fiber composite materials, in particular to a flame-resistant high-performance fiber composite material and a preparation method thereof.The flame-resistant high-performance fiber composite material is prepared from, by weight, 100 parts of polyamide resin, 30-60 parts of composite fibers, 0.4-0.5 part of an antioxidant, 10-18 parts of a flame retardant, 1-2 parts of an interface compatilizer, 0.5-1 part of a lubricant and 0.5-1 part of zinc borate; the flame retardant is prepared from diatomite, aluminum diethylphosphinate and polyphosphamide; the composite fibers comprise modified basalt fibers, phenolic fibers and polyimide fibers; the flame-retardant high-performance fiber composite material prepared by the preparation method disclosed by the invention is good in flame retardant property.

The present invention belongs to the technical field of biochemistry, and specifically relates to a phosphoramidite lipid molecule and a use thereof in an RNAi molecule. In the present invention, a series of lipid molecules containing phosphoramidite are prepared and reacted with oligonucleotides to prepare an RNAi molecule. The RNAi molecule can effectively target extrahepatic tissues, while not interfering with the effect of inhibiting a target gene. The lipid molecule prepared in the present invention has good prospects in extrahepatic delivery vectors, and the RNAi molecule prepared in the present invention has important value for the research and development and clinical application of RNAi drugs.