The invention relates to a brush-coating LED transparent primer, and a preparation method and application thereof, and belongs to the field of chemical coatings; the brush-coating LED transparent primer comprises the following components in parts by weight: 18-30 parts of amino acrylic resin, 30-20 parts of polyester acrylic resin, 3-5 parts of bifunctional aliphatic polyurethane acrylic resin, 0.3-1 part of a defoaming agent, 0.3-1 part of a dispersant, 25-40 parts of an acrylate monomer, 0.5-1 part of fumed silica, 5-15 parts of a photoinitiator, 3-5 parts of an acrylate monomer dissolved CAB solution, 0.5-1.5 parts of a polishing auxiliary agent, 0.05-0.15 part of a base material wetting agent, 0.05-0.15 part of a leveling agent and 0.1-0.3 part of an anti-settling auxiliary agent. Thepreparation method comprises the steps: stirring the amino acrylic resin, the polyester acrylic resin, the bifunctional aliphatic polyurethane acrylic resin, the defoaming agent, the dispersing agentand the photoinitiator; adding fumed silica and the polishing auxiliary agent for dispersion; and adding the rest raw materials and stirring at low speed. According to the invention, the problems of poor surface drying, easy sagging during brush-coating construction and the like caused by oxygen inhibition during brush-coating transparent primer curing are solved, and the environmental protectionproperty during painting is improved.