The invention relates to bread and a preparation method thereof, in particular to seafood and potato nutritional bread and a preparation method thereof. The seafood and potato nutritional bread is prepared from, by mass, 75-100 parts of mashed potato, 20-40 parts of minced seafood, 5-10 parts of algae powder, 1-2 parts of dried yeast, 0.1-0.5 part of glutamine transaminase, 2-5 parts of glucoamylase, 3-6 parts of vital gluten, 1-4 parts of table salt, 16-25 parts of white granulated sugar, 7-12 parts of vegetable oil, 5-10 parts of butter and 40-60 parts of water. The preparation method includes the steps of minced seafood preparation, yeast activation, dough making, proofing, forming, dough fermentation and roasting. The seafood and potato nutritional bread is unique in flavor, rich in nutrition and easy to prepare; the flavor and quality of the potato product are improved, so that the acceptance of consumers is improved, the consumption market of potatoes is expanded, and the national strategic deployment of using potatoes as staple food is further responded to.