The invention discloses an active ionophore powder--Weikangsu powder prepared from natural ores and preparation and application methods thereof. The Weikangsu powder contains stone needle, stalactite, amethyst, calcite, amber, actinolite, agate, mica and an alkaline solution. The preparation method comprises the following steps: weighing the ores in parts by weight and respectively crushing the ores into powders; filling the crushed powders into the alkaline solution and putting into a reaction kettle, heating, vacuumizing to prepare a mixed liquid; preparing a filtrate; and centrifuging to extract a centrifugate, pouring the centrifugate into vacuum freeze drying equipment, freezing, and performing vacuum ultrasonic mixing freeze drying to obtain the active ionophore powder--Weikangsu powder. Distilled water is injected into the Weikangsu powder in application, then traditional Chinese medicine components are added to prepare sprays of Tonglimei, Xianbikang, Rubikang, Zubikang, Xinbikang, Liebikang and Ganbikang for quickly treating various diseases such as soft tissue pain, psoriasis, hyperplasia of mammary glands, dermatophytosis, angina, chronic prostatitis, influenza and the like, and the Weikangsu powder is also widely used in the tobacco industry, the chemical industry and the like.