The invention discloses a novel crystal form I of butorphinol tartrate. An X-ray powder diffraction pattern represented by a diffraction angle of 2 theta +/-0.2 degrees has characteristic peaks at the positions of 10.42 degrees, 12.66 degrees, 14.72 degrees, 16.08 degrees, 20.18 degrees, 21.70 degrees, 23.42 degrees, 24.46 degrees, 25.28 degrees, 27.30 degrees and the like. According to the crystal form I disclosed by the invention, a water-containing crystallization solvent system is adopted, an excessive alcohol solvent is adopted to dissolve butorphinol free alkali, tartaric acid is dissolved by water, feed liquid is not easy to separate out in a filter pressing operation process of entering a clean area, and industrial amplification operation is utilized, so that the purity of the crystal form I is greatly improved. Meanwhile, the obtained crystal form has the advantages of being high in crystallinity, good in crystal form stability and beneficial to production and storage, in addition, water and ethanol of three solvents are used as a crystallization solvent, and the crystal form has the advantages that the quality of a finished product is easy to control, and the risk is low.