The invention provides an organic light-emitting device comprising a conductive anode substrate, a hole injection layer, a hole transport layer, a luminescent layer, an electron transport layer, an electron injection layer and a composite cathode layer. The above-mentioned layers are successively laminated. The composite cathode layer includes a first metal layer, a doping layer, and a second metal layer, wherein the layers are successively laminated. Materials used by the first metal layer and the second metal layer are selected from silver, aluminum, platinum or gold; the doping layer is made of a mixing material formed by an organosilicone micromolecule material doped with VB group metallic oxide, wherein the organosilicone micromolecule material can be diphenyl di (o-tolyl) silicon, p-di(triphenylsilyl) benzene, 1,3-bis(triphenylsilyl)benzene or p-bis (triphenylsilyl)benzene and the VB group metallic oxide can be tantalum pentoxide, vanadium pentoxide, or niobium pentoxide. Because of the composite cathode layer, the light can be scattered, thereby improving the luminous efficiency of the device. In addition, the invention also provides a preparation method of the organic light-emitting device.