4 results about "Extinction spectrum" patented technology

The present disclosure provides an integrating-sphere-assisted resonance synchronous (ISARS) spectroscopy method performed with an ISARS spectrophotometer, for example, a spectrofluorometer equipped with an integrating-sphere accessory. The methods and systems of the present disclosure utilize the ISARS spectrophotometer equipped with an integrating sphere to determine the ISARS spectral intensity, quantify the ISARS-based absorbance, and subsequently evaluate the sample's double-beam UV-vis absorbance spectrum (also known as the absorption extinction spectrum). This ISARS method enables quantitative separation of light absorption and scattering contribution to the sample UV-vis extinction spectrum measured with double-beam UV-vis spectrophotometer.

The present invention relates to a method for quantitative analysis of a plurality of components in at least one aqueous sample comprising an active pharmaceutical ingredient (API), the method comprises the steps of (a) providing at least one aqueous sample comprising a plurality of components, wherein the sample comprises a biologic API; (b) measuring the extinction spectrum of the at least one aqueous sample using a Fourier transform infrared (FT IR) spectrometer comprising a transmission flow-through cell in a single measurement; (c) providing at least one reference extinction coefficient spectrum for each component of the plurality of components to be analyzed in the at least one aqueous sample; and (d) quantifying the concentration of each component in the plurality of components to be analyzed in the at least one aqueous sample based on the reference extinction coefficient spectra of step (c) and the extinction spectrum of the at least one aqueous sample of step (b) by solving the classical least square (CLS) problem by an algorithm using a numerical linear algebra method for finding the approximate solution to an overdetermined system of linear equations characterized by the Beer-Lambert law for the extinction spectrum of the at least one aqueous sample. The invention further relates to methods for the production of an API or determining the purity of an API comprising using said method.

The present application relates to a kind of particle size and mixing ratio measurement method, device, electronic equipment and storage medium, comprising: by extinction method measurement to the two-phase system mixed with first solid particles and second solid particles obtains corresponding experimental extinction spectrum, first solid particles and second solid particles are two different types of solid particles.By calculating the extinction coefficient of first solid particles and second solid particles under the action of light wave is obtained.By calculating the albedo of first solid particles and second solid particles, and judging whether corresponding photon is scattered.If photon is scattered, the scattering angle when photon is scattered and the random free step length between adjacent two scattering of photon are obtained.According to the direction of photon exit, the corresponding theoretical extinction spectrum of the two-phase system mixed with first solid particles and second solid particles is obtained by calculation.Based on experimental extinction spectrum and theoretical extinction spectrum, the particle size and mixing number ratio of solid particles are obtained.

The invention provides a method and system for detecting extinction, scattering and absorption characteristics of noble metal nanoparticles, and relates to the technical field of nano optical detection.The method comprises the steps that a sample with single particle distribution is prepared, the dark field scattering spectrum of a single particle is collected under dark field illumination, and the characteristic resonance wavelength of the single particle is determined through pretreatment and formant recognition; switching to bright field illumination, collecting a transmission spectrum at the same position, calculating an extinction spectrum and combining the extinction spectrum with scattering intensity to determine an absorption intensity initial value at a characteristic resonance wavelength; collecting a photo-thermal signal of the particle, and correcting the initial value of the absorption intensity by taking the amplitude of the photo-thermal signal as a correction factor to obtain a corrected absorption intensity value; according to the scattering intensity and the corrected absorption intensity value, the scattering efficiency and the absorption efficiency at the characteristic resonance wavelength are calculated, and then a scattering-absorption adaptation coefficient is determined; and comparing the adaptation coefficient with a dominant threshold, and judging that the nanoparticles are suitable for scattering-dominant, absorption-dominant or scattering-absorption synergistic applications.