12 results about "Lactone synthesis" patented technology

The invention discloses a thermostable conjugated polyketoreductase mutant, which is obtained by performing single-point mutation or multi-point combined mutation on the 25th site, the 92th site, the 214th site or the 256th site of an amino acid sequence as shown in SEQ ID NO.1. The invention further discloses a preparation method of the thermostable conjugated polyketoreductase mutant. The initial enzyme activity of the mutant is high, and the residual enzyme activity is gt after incubation at 50 DEG C; the Tm value can be increased by more than 9 DEG C compared with that of an original strain, the 50 DEG C half-life period t1 / 2 is increased by more than 377.8 times, and the thermal stability is obviously improved. The invention also provides a coding gene, a recombinant vector containing the coding gene and a co-expression engineering bacterium. The thermostable conjugated polyketoreductase mutant, glucose dehydrogenase and L-pantolactone dehydrogenase are used for constructing a three-enzyme co-expression engineering bacterium as a biocatalyst, racemization pantolactone is catalyzed to synthesize DPL, for a 1M substrate, the yield of the DPL reaches 97.5% within 36 hours, KPL hydrolysis is avoided, the reaction time is shorter, the product concentration is higher, and the thermostable conjugated polyketoreductase mutant has great market application value.

The invention discloses a gene GbCYP701A6 for promoting synthesis of ginkgo terpene lactones and application of the gene GbCYP701A6. The nucleotide sequence of the gene GbCYP701A6 is shown as SEQ ID NO.1, and the amino acid sequence of protein expressed by the gene GbCYP701A6 is shown as SEQ ID NO.2. The invention further discloses a preparation method of the gene GbCYP701A6. According to the invention, a brand new gene GbCYP701A6 is cloned from gingko for the first time. The GbCYP701A6 gene is transferred into a ginkgo biloba body, the GbCYP701A6 gene is over-expressed, the contents of terpene lactones including ginkgolide A, ginkgolide B, ginkgolide C and bilobalide in ginkgo biloba are remarkably increased, and the contents of the ginkgolide A, ginkgolide B, ginkgolide C and bilobalide are remarkably reduced when the gene is silently expressed, so that the gene is a key gene for promoting synthesis of the ginkgo terpene lactones, and the GbCYP701A6 gene can be used for preparing the ginkgo biloba terpene lactones. Therefore, the regulation and control of the expression of the GbCYP701A6 has important application value in the aspects of improving the medicinal quality of the ginkgo leaves and the like.

The present invention provides a new alpha-chloro-alpha-acetyl-gamma-butyrolactone synthesis process, and relates to the technical field of alpha-chloro-alpha-acetyl-gamma-butyrolactone synthesis, and the new alpha-chloro-alpha-acetyl-gamma-butyrolactone synthesis process comprises the following steps: mixing water, alpha-acetyl-gamma-butyrolactone and baking soda, introducing chlorine gas to carry out a reaction, then removing the chlorine gas, and after the reaction is completed, filtering the reaction liquid to obtain the alpha-chloro-alpha-acetyl-gamma-butyrolactone. Then, standing for layering, so as to obtain alpha-chloro-alpha-acetyl-gamma-butyrolactone and chloro ester layered water; and the chlorine ester layered water jacket is used for the next batch reaction to synthesize the alpha-chloro-alpha-acetyl-gamma-butyrolactone. According to the method, the steam energy consumption of triple-effect evaporation salt elimination of the chlorine ester layered water can be reduced, about 1% of chlorine ester dissolution loss in the chlorine ester layered water can be avoided, the cost advantage of the product is effectively improved, organic wastewater generated by distillation can be reduced, and the wastewater treatment load of a biochemical system can be reduced.

The present invention relates to the chemical synthesis of cannalactone, an adaptation of this synthesis leading to deuterium labeling of cannalactone, as well as the use of deuterium-labeled cannalactone to perform the dosage of natural cannalactone of the exudates or tissues of the hemp plant.

The invention discloses application of a DNA methylation inhibitor in regulation and control of synthesis of ginkgo terpene lactones in the technical field of plant cultivation, and the epigenetic regulation and control effect of 5-azacytidine on leaf growth and terpene lactones synthesis is researched by taking ginkgo as a material. 5-AzaC with different concentrations is set for treatment, and the dynamic change of leaf growth traits and terpene lactone accumulation is systematically analyzed. Researches show that treatment of 5-AzaC shows a significant dose effect: low concentration (50 [mu] mol / L) promotes increase of fresh weight and increase of terpene lactone content, and high concentration (greater than or equal to 150 [mu] mol / L) inhibits growth but significantly increases accumulation of bilobalide and ginkgolide C. Correlation analysis shows that biomass and terpene lactone content are in significant negative correlation, a growth and defense balance phenomenon is proved, the invention reveals that 5-AzaC affects synthesis and accumulation of different terpene lactones by regulating DNA methylation level difference, and a theoretical basis and a technical path are provided for accurate regulation of ginkgo medicinal components.

The present application relates to the technical field of delta-lactone synthesis, in particular to a high-purity delta-lactone synthesis method, comprising the following steps: using potassium carbonate to catalyze Michael addition reaction of the active methylene functional group of dimethyl malonate and the alpha, beta-alkenyl functional group of 1-octene-3-ketone to generate intermediate product I; intermediate product I generates decarboxylation reaction under the catalysis of boric acid to generate intermediate product II; chiral ligand supported nickel catalyst catalyzes asymmetric hydrogenation reaction of the delta-carbonyl functional group of intermediate product II to generate chiral delta-hydroxyl functional group, and ester exchange reaction of the chiral delta-hydroxyl functional group and the ester functional group of intermediate product II to generate chiral delta-decalactone. The present application designs and synthesizes four chiral ligand supported nickel catalysts on one hand, and designs a new route capable of synthesizing chiral delta-decalactone with high optical purity on the other hand.

The invention discloses a gene GbSAUR48 for promoting synthesis and biomass of bilobalide as well as a carrier, a protein and application of the gene GbSAUR48. The nucleotide sequence of the gene GbSAUR48 is shown as SEQ ID NO. 1. According to the invention, the GbSAUR48 gene specifically and highly expressed in the ginkgo biloba root reveals dual functions of the GbSAUR48 gene in promoting lateral root generation, improving overground and underground biomass and promoting bilobalide synthesis for the first time. The number of fibrous roots / lateral roots and the overall overground / underground biomass of the plant over-expressed with GbSAUR48 are remarkably increased, and meanwhile the content of active terpene lactones (GA and GB) is synchronously increased. The invention not only shows that the GbSAUR48 is a key gene for regulating and controlling the quality (medicinal quality) and yield (biomass) of the gingko, but also provides a new theoretical basis and a practical path for realizing double improvement of the medicinal value and the yield of the gingko through a molecular breeding means.

The application discloses a conjugated polyketide reductase mutant, which is obtained by single-point mutation or multi-point combined mutation of the 64th or 206th site of the amino acid sequence shown in SEQ ID NO. 1. The mutant of the conjugated polyketide reductase can improve the enzyme activity by more than 25% compared with the wild type. The application also provides a coding gene, a recombinant vector containing the coding gene, a co-expression engineering bacterium and application. The conjugated polyketide reductase mutant, glucose dehydrogenase and L-panthenolide dehydrogenase are used to construct a three-enzyme co-expression engineering bacterium as a biological catalyst to catalyze the synthesis of optically pure D-panthenolide from racemic panthenolide, improve the catalytic efficiency of multi-enzyme cascade reaction, reduce the hydrolysis loss of intermediate product KPL, and significantly improve the yield of DPL. The yield of the obtained DPL can be up to 98%, and the optical purity can be up to 99.9%.

The invention provides a construction method and application of a costunolide biosynthetic pathway, and the construction method comprises the following steps: co-expressing four genes and seven genes for converting acetyl coenzyme A into FPP in escherichia coli to form a mevalonic acid pathway, and constructing the costunolide biosynthetic pathway; the four co-expressed genes comprise an NS gene for coding germacene A synthetase, a CiGAO gene for coding germacene A oxidase, a CiCOS gene for coding costunolide synthetase and a CPR gene; the seven genes for converting the acetyl coenzyme A into the FPP comprise mvaE, mvaS, mvk, pmk, mvaD, IDI and ispA; according to the invention, the costunolide yield of 20.82 mg / L is realized in a shake flask, and is increased by 274 times compared with that of an initial strain.

The utility model discloses a stirring and dropwise adding device for synthesis of methyl fenugreek lactone, which belongs to the technical field of food additive production and comprises a mixing tank, a storage tank communicated with the top of a connecting pipe, a dropper fixedly connected with the bottom of a rotating frame, an adjusting opening formed in the inner bottom of the rotating frame through the dropper, and a stirring device connected with the adjusting opening. A telescopic rod is fixedly connected to the inner bottom of the rotating frame, a moving plate is fixedly connected to the top of the telescopic rod, and an adjusting block is fixedly connected to the center of the bottom of the moving plate. According to the stirring and dropwise adding device for synthesis of the methylfenugreek lactone, the stirring assembly drives the dropwise adding assembly to rotate, so that materials can be uniformly dropwise added in the mixing tank, non-uniform dropwise adding is effectively prevented, a telescopic rod mounted in a rotating frame drives a moving plate and an adjusting block to move, and the stirring assembly is used for driving the dropwise adding assembly to rotate; an adjusting block enters an adjusting opening to adjust the dropwise adding rate and the dropwise adding amount, so that the dropwise adding speed of the material can be accurately controlled, and the synthesis reaction is effectively prevented from being too violent or slow.

The application provides a method for synthesizing 2-(3-hydroxypropyl)cyclododecanone, which comprises the following steps: taking cyclododecanone as a starting material, synthesizing cyclododecanone diallyl acetal with diallyl acetal, and then obtaining 2-(3-hydroxypropyl)cyclododecanone through a cleavage reaction. The addition of palladium metal compounds in the reaction process effectively reduces the addition products of cyclododecanone and multiple allyl alcohols, and the coupling products of cyclododecanone itself, so that the effect of inhibiting side reactions is achieved. 2-(3-hydroxypropyl)cyclododecanone is an important intermediate for synthesizing cyclopentadecanolide, solves the problem of limited raw materials in the synthesis route of the musk cyclopentadecanolide, and has the advantages of high atom utilization rate and low raw material cost.

The present application provides an L-pantoic acid lactone dehydrogenase derived from Skermania sp. ID1734, a nucleic acid molecule encoding the L-pantoic acid lactone dehydrogenase, a vector for expressing the L-pantoic acid lactone dehydrogenase in a host cell, and an engineered bacterium expressing the enzyme. The present application further provides an engineered bacterium co-expressing the L-pantoic acid lactone dehydrogenase and any one or more selected from the group consisting of a ketopantoate reductase and a glucose dehydrogenase. The present application further provides a method for producing D-pantoic acid lactone using an engineered bacterium expressing the L-pantoic acid lactone dehydrogenase derived from Skermania sp. ID1734. One of the technical solutions provided by the present application is to provide a recombinant vector or a combination of recombinant vectors, wherein the recombinant vector or the combination of recombinant vectors comprises a gene expressing the L-pantoic acid lactone dehydrogenase. The present application also provides the use or application of the L-pantoic acid lactone dehydrogenase, the nucleic acid molecule, the engineered bacterium, the vector or the combination of vectors in the synthesis of D-pantoic acid lactone.