The invention discloses an annular permanent magnet low frequency vibration isolation mechanism based on a negative stiffness theory. The mechanism comprises an upper rubber sheet, an inner annular permanent magnet fixing mandrel, an upper rubber sheet upper pressing ring, an upper rubber sheet outer pressing ring, an outer annular permanent magnet, a lower rubber sheet, a lower rubber sheet inner pressing sheet, a lower rubber sheet outer pressing ring, an outer annular permanent magnet fixing sleeve and an inner annular permanent magnet, wherein the inner annular permanent magnet is magnetized axially; the outer annular permanent magnet is radially magnetized; a positive stiffness system consists of the inner annular permanent magnet and the outer annular permanent magnet; a negative stiffness system consists of the upper rubber sheet and the lower rubber sheet; and the positive stiffness system and the negative stiffness system are used in parallel to form the low frequency vibration isolation mechanism. Three or four single-degree-of-freedom vibration isolation mechanisms are used in parallel to realize three-degree-of-freedom low frequency vibration isolation. When the vibration isolation mechanism has the largest magnetic force, the stiffness of the vibration isolation mechanism approximates to zero. Without an outside air source, the vibration isolation mechanism has a prospect of being used in vacuum, has the characteristics of simple structure and low cost, is easy to process, and can be applied to the fields of optics, acoustics, biologics, semiconductor manufacturing, precise measurement and the like.