The invention discloses a kit for detecting a biomarker of an Alzheimer's disease, and relates to the technical field of in vitro diagnosis. The kit comprises a horse radish peroxidase labeled biomarker antibody solution, an anti-biomarker antibody coated elisa plate, a concentrated washing solution, a luminous substrate solution, a calibration product and a quality control product. The inventionfurther discloses a preparation method of the elisa plate coated with the anti-biomarker antibody. The kit has the beneficial effects that on the basis of chemiluminiscence immunoassay, the elisa plate coated with the anti-biomarker antibody is introduced, a coating process of the anti-biomarker antibody is improved, the reaction sensitivity is increased, a reagent production process is simplified, production time is shortened, and the detection kit which is high in quality, low in price, stable, reliable, good in repeatability, small in batch difference and high in accuracy is provided for the market.