5results about How to "Solve key technical problems" patented technology

The application provides application of glutaroyl-CoA dehydrogenase in inducing ferroptosis of liver cancer cells, and belongs to the technical field of biotechnology.The application provides application of glutaroyl-CoA dehydrogenase (GCDH) in preparing products for evaluating risk of liver cancer and evaluating prognosis risk of liver cancer, and the tumor stage and T grade can be estimated by detecting the expression amount of GCDH, and the survival effect of liver cancer patients is evaluated.The application provides application of the overexpression reagent of GCDH in preparing products for inhibiting proliferation of liver cancer cells and inducing ferroptosis of liver cancer cells, and the ferroptosis of liver cancer cells is promoted by overexpressing GCDH in cells, and the colony formation ability of liver cancer cells is reduced.Further, the application provides application of the overexpression reagent of GCDH combined with a CA9 inhibitor in preparing products for inhibiting proliferation of liver cancer cells.

The invention relates to the technical field of block chain fragmentation, and discloses a pool optimization propagation account division method for a fragmented block chain. The method comprises the steps of obtaining a block chain account set and transaction data, and constructing an account transaction undirected graph adopting a quintuple edge structure; establishing a multi-objective optimization function for simultaneously optimizing cross-fragment transaction frequency, load balance and fund pool balance; constructing a mobile candidate fragment list for each account, and expanding the highly connected accounts to all fragments; multi-thread parallel computing is adopted to evaluate a movement score, movement is executed according to a greedy strategy, and the fragmentation state is updated; and carrying out iterative optimization to obtain an optimal division scheme, and deploying the optimal division scheme to a fragmented block chain system to realize account dynamic allocation. According to the method, the problems that an existing algorithm is prone to local optimum and the fund pool management efficiency is low are solved, the low cross-fragmentation rate and fund flow optimization are achieved while load balance is guaranteed, and the transaction processing success rate and the overall performance of the fragmentation block chain system are improved.

The invention discloses a grid line intersection point positioning method based on connected domain analysis and Gaussian fitting. Relates to the technical field of machine vision and image processing. The method comprises the following steps: acquiring a grid image and preprocessing the grid image to obtain a pure grid image; performing edge feature enhancement and extraction on the pure grid image to obtain an optimized edge grid unit region image; performing connected domain analysis, sorting and angular point identification on the optimized edge grid unit region image to obtain an edge grid unit region total sequence diagram; establishing a region association mapping relationship for the edge grid unit region total sequence diagram; gaussian fitting accurate positioning is carried out based on the regional association mapping relation, and intersection point coordinates are obtained; and obtaining optimized grid intersection point coordinates by solving analysis intersection points of adjacent horizontal grid lines and vertical grid lines. The method can effectively improve the extraction precision and reliability of the grid intersection point coordinates, and is suitable for various image measurement and analysis tasks needing accurate grid positioning.

This invention discloses an individual identification method and system based on whole-genome single nucleotide polymorphism (SNP) data, belonging to the field of forensic genetics technology. The specific steps are as follows: obtaining the intersection SNP sites of the sample pairs to be identified, constructing a linkage-equilibrium SNP site set; evaluating the individual identification ability of the linkage-equilibrium SNP site set based on a power evaluation index, generating an optimal SNP site set; under the set mutual exclusion hypothesis, calculating the probability value of each optimal SNP site based on the population genotype frequency and the prior genotyping error rate, and calculating the total likelihood ratio based on the probability values ​​of each optimal SNP site; selecting a supporting hypothesis or hypothesis based on the relationship between the total likelihood ratio and a preset threshold. This invention, by introducing a semi-continuous likelihood ratio calculation model that integrates the genotyping error rate, can scientifically handle and explain the unavoidable genotyping errors in whole-genome sequencing data.

The invention belongs to the technical field of crystal material preparation, and particularly relates to a method for growing sapphire crystals by a wide seed crystal edge-defined film-fed crystal growth method, which comprises the following steps of: firstly, preparing an Al2O3-based raw material through multi-step sintering, carrying out acid etching microstructuring treatment on R / M surface seed crystals, arranging an array type feeding seam mold above a crucible during charging, and carrying out vacuum replacement for three times, so as to obtain a sapphire crystal; a gradient temperature field is established through three-area independent temperature control, seeding is performed after melt is immersed into a mold, the growth process comprises seeding, shouldering and equal-diameter growth, wide diameter expansion and equal-diameter growth of crystals are realized by accurately controlling the pulling rate, the crucible rising speed and the three-section power of a middle heating area, and the crystals are automatically separated from the mold; and high-temperature, medium-temperature and low-temperature three-stage gradient annealing is carried out under argon protection to release stress and stabilize the structure, so that the transverse temperature gradient can be accurately controlled below 5 DEG C / cm, the problem of large temperature difference between the edge and the center during wide seed crystal growth is solved, and the stability of the solid-liquid interface shape and the uniformity of crystal growth are ensured.