The invention discloses a probiotic-containing hot pot seasoning prepared from the following raw materials: raw ginger, garlic, onion, illicium verum, longan aril, nutmeg, hawthorn, dandelion, liquorice, Sichuan pepper, lotus, black pepper, perila seeds, table salt, Chinese yam, radix puerariae, chili pepper, edible vegetable oil, beef tallow, and a probiotic agent. The invention also discloses apreparation method of the probiotic-containing hot pot seasoning. The prepared hot pot seasoning has the advantages of delicious taste, mellow fragrance, unique flavor and balanced nutrition, containsno edible essence, ensures the natural taste of the hot pot seasoning, meets people's pursuit on natural health, and has broad market prospects.