The invention discloses a preparation process of a health-care summer sleeping mat. The preparation process comprises the steps of cutting, scraping linen, cleaning, drying in the air, degumming, cutting fibers, preparing fibers, splitting fibers, combing fibers, drawing, twisting yarns, obtaining warp yarns and knitting, wherein fiber raw materials consist of pineapple leaf fibers and other textile fibers, the percentage of the pineapple leaf fibers is 40 to 60 percent, the percentage of pineapple head disordered fibers is 30 to 45 percent, and the percentage of textile filler is 10 to 20 percent. The summer sleeping mat prepared by selecting the pineapple leaf fibers as one raw material has the functions of coolness, moisture absorption and discharge, ventilation property, softness, comfortableness, no prodding and itching feeling and the like, has a better effect of repelling and preventing mites, and has the advantages of higher softness, smoothness, washing resistance, storage resistance and durability; the detected summer sleeping mat has the efficacy of naturally resisting bacteria, inhibiting bacteria, repelling mites, removing odor, deodorizing and the like; a sleep environment can be remarkably improved.