The invention relates to a fresh-enhancing and fragrance-enhancing beef seasoner, which is prepared from the following raw material ingredients including acetic acid, 3-hydroxyl-2-butanone, butyric acid, 3-sulfydryl-2-butanone, 3-sulfydryl-2-butanol, 2-methyl-3-sulfydryl furan, 3-methylthiopropionaldehyde, 3- methylthio propanol, 2-acetylthiazole, 2-acetylpyrazine, methyl cyclopentenolone, 2-acetyl pyrrole, furanone, methyl maltol, ginger essential oil, methyl (2-methyl-3-furyl) disulfide, ethyl maltol, anisic aldehyde, cassia oil, sulfurol, 4-ethyl guaiacol, star anise oil, acetic acid sulfurol, difurfuryl sulfide, bi(2-methyl-3-furyl) disulfide and a carrier. After the enhancement, the soft and constant fragrance is generated; the defects of natural beef are overcome; the fishy ingredients are removed; the beef flavor and the beef feeling are remained; when the fresh-enhancing and fragrance-enhancing beef seasoner is applied to food and seasoners, especially beef intestines and beef pills, the fragrance is richer.