The invention discloses a polyester chiffon printing and dyeing process. The polyester chiffon printing and dyeing process comprises pretreatment, dyeing treatment and posttreatment, wherein the dyeing treatment comprises the following steps: putting polyester chiffon into a low-concentration dying solution, performing dip dyeing, taking out the polyester chiffon, directly putting into a high-concentration dying solution, performing dip dyeing, and after naturally cooling the high-concentration dying solution, taking out the polyester chiffon to finish dyeing; the posttreatment comprises the following steps: drying the dyed polyester chiffon, putting into a mixed solution of a softening agent and a color fixing agent, soaking, drying, putting into a soaping box, cleaning, then drying and warehousing; the low-concentration dying solution comprises the following raw materials: dye, a cationic surfactant, a dispersant and cyclohexane; the high-concentration dying solution comprises the following raw materials: dye, a cationic surfactant, a dispersant, a color fixing agent and deionized water. The polyester chiffon printing and dyeing process is simple; by the polyester chiffon printing and dyeing process, the polyester chiffon is dyed under the conditions of room pressure and low temperature, is high in dyeing rate, is uniformly dyed, and is high in fastness, good in softness and antistatic.