The invention discloses the application of a divalent iron supply liquid in promoting the fermentation of the flora used to treat petroleum residues. The flora used to treat petroleum residues includes the following genera: Methanobacterium, Rhizopus, Trichoderma, Pseudomonas bacteria, Aspergillus, Penicillium, Rhodopseudomonas, Lactobacillus, photosynthetic bacteria and yeast; the ferrous iron supply liquid is a mixed solution containing ferrous ions, and the mixed solution Contains sodium ferrous citrate, ferrous sulfate, ferrous citrate, the molar mass ratio of sodium ferrous citrate, ferrous sulfate, ferrous citrate in the mixed solution is 1:0.28~0.38:0.17~0.23, the two The pH of the valent iron supply liquid is 4.5-5.5. In the present invention, the ferrous iron supply liquid can promote the growth and development of the flora, accelerate the metabolism of the flora, and accelerate the fermentation and decomposition of petroleum residues by the flora, which can greatly shorten the fermentation cycle, increase the volume reduction rate, and at the same time reduce the heavy metal Passivate.