The invention discloses an ointment for treating rotavirus enteritis. The ointment is prepared from the following raw material medicines: 10-15g of agastache rugosus, 12-18g of rhizoma atractylodis, 10-15g of pericarpium citri reticulatae, 10-15g of fructus amomi, 28-32g of poria cocos, 12-18g of cortex magnoliae officinalis, 12-18g of pericarpium arecae, 22-26g of rice sprouts, 28-32g of coix seeds and 12-18 g of myrobalan pulp. The invention also provides a preparation method of the ointment. The ointment disclosed by the invention is applied to the Shenque acupoint, the spleen acupoint andthe stomach acupoint after being heated, so that the ointment plays a role in treatment through direct subcutaneous absorption and indirect stimulation to stimulate meridian Qi of the acupoints. Aftertreatment, abdominal pain, diarrhea, nausea, vomiting, inappetence, abdominal distension, tiredness, fatigue and the like of a sick child are obviously superior to those of oral montmorillonite powder, and are obviously superior to those of a Dingguier navel patch in the aspects of comprehensive curative effect, syndrome curative effect, RV negative conversion rate and the like. The ointment is remarkable in curative effect, convenient to use, good in safety, low in price, simple in medicine and high in clinical popularization value.