The invention discloses a glass microsphere for a TPU reflecting film and a preparation method of the glass microsphere. The glass microsphere is prepared from such raw materials in parts by weight: 25-35 parts of sepiolite, 36-49 parts of glass cullet, 22-38 parts of variscite, 16-27 parts of rutile, 22-36 parts of leucite, 30-40 parts of gold tailings, 5-10 parts of polymerized alpha-olefin, 3-6 parts of sorbitan olivate, 2-4 parts of dodecafluoropropyltrimethoxysilane, 3-6 parts of ethoxylated castor oil, 4-8 parts of 1,4-butanesultone and 8-14 parts of additives. Due to reasonable proportioning and scientific preparation process and technique, the production of high-quality, high-refractive index and high-stability of glass microspheres is realized; besides, the glass microspheres have good mechanical properties, corrosion resistance, weather resistance and high temperature resistance; furthermore, large-scale batch production can be realized.