The invention discloses spicy beef sauce. The spicy beef sauce is prepared from the following raw materials: beef, peppers, onions, vegetable oil, salt, paprika powder, star aniseed powder, fresh ginger powder, soy sauce, oyster sauce, sauce and water. A preparation method of the spicy beef sauce comprises the following steps of S1, conducting pretreatment on the beef; S2, immersing the pre-treated beef in water containing sodium chloride and in sauce for removing the smell of the beef to obtain the smell-removed beef; S3, adding a softening agent in the smell-removed beef, placing the beef ina sealed place, peeling off the softening agent, and rinsing with fresh water to obtain the softened beef; S4, washing and dicing the peppers and the onions; S5, adding the vegetable oil into a pan,stir-frying pepper dices, onion dices and ingredients, adding the beef and stir-frying, adding soy sauce and oyster sauce and stir-frying, and finally adding sauce and water to boil until the viscosity is suitable, so that the spicy beef sauce is obtained. The spicy beef sauce prepared by the method has reasonable formula, good taste, easy chewing and wide applicable population, spicy taste and beef taste complement each other, and the preparation method is simple in process, mild in conditions and short in preparation time.