The invention discloses a method for increasing the survival rate of adults of live oreochromis niloticus. The hormone level, the alexin level and the hepatic antioxidant adaptive capacity can be improved within a short time by increasing breeding density and performing tensioning net stress, and nonspecific immunity is improved. The contents of protein, glucose and triglyceride in blood of the oreochromis niloticus can be increased by feeding feedstuff with high protein level of 36%-39% before the oreochromis niloticus is conveyed, and energy reserve is improved. Moreover, 0.5%-0.8% of compound heartleaf houttuynia herb is added in the feedstuff and contains herba houttuyniae, scutellaria baicalensis, radix isatidis, fructus forsythiae, honeysuckle and the like, and the anti-stress capability of the oreochromis niloticus can be improved. In a conveying process, the water temperature is controlled in a range of 19-23 DEG C, the salinity of water is 8%o-12%o, and dissolved oxygen is sufficient. The metabolic activity of the oreochromis niloticus is low, but the immunity of the oreochromis niloticus is high, so that energy loss in the conveying process is reduced, excretion of metabolin is reduced, and the anti-stress capability is improved. After the oreochromis niloticus is treated by using the method, the survival rate of the adults of the live oreochromis niloticus and the live oreochromis niloticus conveying distance are obviously improved during conveyance.