The invention discloses a biological source food preservative material composition, a biological source food preservative and a preparation method thereof. The biological source food preservative material composition is prepared from nisin, chitosan, garlic, ginger, Chinese prickly ash, cloves, Chinese cinnamon, nutmegs, star aniseed, fennel, honeysuckle flowers, argy wormwood leaves, plantain herb, linearstripe rabdosia herb, dandelion herb and an ethanol aqueous solution. By the technical scheme of the invention, the effects of convenient and simple preparation method, wide raw material sources, high utilization rate and good feasibility are realized.