The invention belongs to the technical field of cosmetics and particularly relates to sea urchin polypeptide face cream and a preparation method thereof. The sea urchin polypeptide face cream is prepared from components as follows: sea urchin polypeptides, hemp oil, olive oil, an emulsifier, a moisturizer, a thickener, a preservative and distilled water. The sea urchin polypeptide face cream and the preparation method have the beneficial effects as follows: discarded internal organs of sea urchin processing are subjected to enzymolysis, separation, purification and other treatment, sea urchin polypeptides with molecular size being 1kD-10 kD are extracted and have the characteristics of being free from foreign odor, good in water solubility and easily absorbed by skin, then the sea urchin polypeptides are mixed with other components, the prepared face cream has the advantages of being free from foreign odor and easy to absorb, metabolism of skin cells can be promoted, and skin moisture and elasticity are kept.