Patents
Literature
Hiro is an intelligent assistant for R&D personnel, combined with Patent DNA, to facilitate innovative research.
Hiro

36 results about "Milbemycin oxime" patented technology

Milbemycin oxime (trade name Interceptor, marketed by Elanco) is a veterinary drug from the group of milbemycins, used as a broad spectrum antiparasitic. It is active against worms (anthelmintic) and mites (miticide).

Antibody library of bacteriophages and applications in immunoassay of pesticide residue

The invention relates to a phage antibody library which assembles an ScFv gene fragment between Restriction Enzyme cutting sites of SfiI and NotI of a pCANTAB5E carrier. The phage antibody library is characterized in that the ScFv gene fragment can be affinitive and enriched with antigens of 16-membered macrolide agricultural chemicals to form a soluble single-chain antibody and the phage antibody library is applied to immunoassay of pesticide residue. The phage antibody library has the advantages that the antibody with high affinity can be obtained without animal immunization, the test period is short, the antibody library is the phage antibody library which takes small molecular milbemycin oxime of a 16-membered macrolide generic structure as immunogen to construct, the antibody library theoretically can directly obtain a specific antibody library of 16-membered macrolide compound through screening, the screening flux is high, the efficiency is high, the specificity is strong, and the affinity selecting range is wide, so that the phage antibody library has wide application prospect in the aspects such as agricultural chemical antibody preparation, testing technique development and the like.
Owner:JIANGSU ACADEMY OF AGRICULTURAL SCIENCES

Preparation method of milbemycin oxime

The invention provides a preparation method of milbemycin oxime. The preparation method of milbemycin oxime comprises the following steps: extracting milbemycins, namely taking milbe mycelia obtained through fermentation as a raw material, extracting, concentrating, extracting, concentrating, extracting again and concentrating again, so as to obtain milbemycin; preparing milbemycin ketone, namely taking the milbemycin as a raw material, establishing an oxidation reaction system, after reaction is finished, filtering, concentrating, extracting and concentrating the reaction system, so as to obtain an intermediate product milbemycin ketone; synthesizing milbemycin oxime, namely dissolving milbemycin ketone with methanol and dioxane, dropwise adding hydroxylamine hydrochloride solution and reacting, concentrating the reaction system, extracting, drying and concentrating, so as to obtain a milbemycin oxime crude product; and purifying milbemycin oxime, namely crystallizing the milbemycin oxime crude product with a mixed solvent of trichloromethane and normal heptane, dissolving a crystalline product with ethanol, dropwise adding into water while stirring for carrying out crystallization, filtering, and drying, so that the milbemycin oxime finished product is obtained. The preparation method of the milbemycin oxime has the advantages that productivity is greatly improved, and cost is obviously reduced, so that the preparation method provided by the invention is obviously better than an existing preparation method.
Owner:HUBEI HONCH PHARMA

Milbemycin oxime compound and preparation method thereof

The invention discloses a milbemycin oxime compound and a preparation method thereof. The compound as shown in a general formula (I) in the specification is prepared by the steps of carrying out hydrolysis reaction on avermectin B1a to remove C13-position glycosyl, oxidizing both C5-position hydroxyl and C13-position hydroxyl into keto through oxidation reaction, and finally converting the two kinds of keto into oximido, wherein the structure of the compound is confirmed by virtue of the mass spectrum and nuclear magnetic resonance analysis testing technology. According to the milbemycin oxime compound and the preparation method, the milbemycin oxime compound is prepared by starting materials which are cheap and easily obtained; the preparation method has the characteristics of mild reaction condition, simpleness in operation process, low cost and higher yield.
Owner:WUHAN UNIV

Synthetic method for milbemycin oxime

The invention provides a synthetic method for milbemycin oxime. The method comprises the following steps that 1, an oxidation reaction is performed, wherein milbemycins is used as raw materials, pypocholoride or chlorite is used as an oxidizing agent, piperidines free radical of nitroxide is used as a catalyst, a halide is used as a promoter, all the materials are subjected to the reaction in a dichloromethane solvent at the temperature being minus 5-15 DEG C for 0.5-4 h, and postprocessing is performed on a reaction product to obtain an intermediate product of milbemycin ketone; 2, an oximation reaction is performed, wherein methyl alcohol and 1,4-dioxane are used as a reactive solvent, hydroxylamine hydrochloride is used as an oximation reagent, the reaction is performed at the temperature being 25-35 DEG C for 10-16 h, and postprocessing and purification are performed to obtain milbemycin oxime. According to the method, industrial production of the milbemycin oxime is achieved in China for the first time, and the yield of the obtained product is higher than that of similar products both at home and abroad. Due to the fact that the pypocholoride is used as the oxidizing agent, pyridine nitroxide free radical is used as a catalyst, conditions are mild, few side reactions exist, the yield is high, and the cost is low.
Owner:HUBEI HONCH PHARMA

Milbemycin oxime separation and purification method

The invention provides a milbemycin oxime separation and purification method. The milbemycin oxime separation and purification method comprises the following steps: carrying out crude separation on a sample to be separated through silicagel column chromatography, wherein the silicagel column chromatography adopts wet-process column packing and dry-method sample loading; purifying the product after crude separation by high performance liquid chromatography; concentrating the purified sample by a nanofiltration membrane to obtain concentrated solution; performing reduced-pressure vaporization to the concentrated solution, filtering and drying to obtain a refined product; dissolving the refined product, heating up, dropwise adding isooctyl alcohol, normal heptane or petroleum ether, cooling to be crystallized, and filtering to obtain a crystallized product; dissolving the crystallized product, filtering, dropwise adding filtrate into purified water stirred constantly, after dropwise adding, carrying out suction filtration to obtain a crystal transformation product; drying the crystal transformation product, crushing, and drying again to obtain milbemycin oxime finished product. The method can realize industrial production of milbemycin oxime in China firstly, and the product has high purity and high yield which are higher than those of the similar products at home and abroad.
Owner:HUBEI HONCH PHARMA

Milbemycin oxime and praziquantel flavored tablet and preparation method thereof

The invention relates to a milbemycin oxime and praziquantel flavored tablet and a preparation method thereof. The milbemycin oxime and praziquantel flavored tablet consists of a tablet core and a drug-free coating layer coating the surface of the tablet core, wherein the coating layer can be one or more layers, and the coating layer contains at least one phagostimulant or flavoring agent; and thetablet core consists of pellets, a flow aid and a lubricant. The preparation process of the milbemycin oxime and praziquantel flavored tablet comprises preparation of drug-containing powder, preparation of the pellets, preparation of the tablet core, preparation of the coating layer and preparation of the flavored tablet. The preparation technology is simple, and the main drug and the auxiliary agent can be uniformly mixed together, so that the auxiliary agent can better play a role, the stability of the drug is improved, the taste of the drug is improved, and the milbemycin oxime and praziquantel flavored tablet is suitable for industrial production and has a wide application prospect.
Owner:ZHEJIANG HISUN ANIMAL HEALTH PROD CO LTD

Oily injection containing antiparasitic agent/polyethylene glycol drug-loading microparticles

The invention provides an oily injection containing antiparasitic agent / polyethylene glycol drug-loading microparticles which is prepared by suspending drug-loading microparticles, which are composed of antiparasitic agent and solid polyethylene glycol, in an oily medium. The antiparasitic agent comprises ivermectin, abamectin, eprinomectin, doramectin, milbemycin oxime, moxidectin, albendazole oxide or hydrochloride thereof, oxfendazole or hydrochloride thereof, closantel base or sodium salt thereof; polyethylene glycol with molecular weight of more than 6,000is preferably selected for preparation of the oily injection; and one of isopropyl myristate, injection soybean oil, maize oil and tea seed oil is preferably selected for preparation of the oily injection.
Owner:王玉万

Method for synthesizing milbemycin oxime compound

The object of the invention is to provide a method for synthesizing a milbemycin oxime drug. The implementation method provided by the invention comprises the steps: subjecting one or the mixture of avermectin B1a and avermectin B1b, which serves as a raw material, to a hydroxyl protecting reaction so as to protect a hydroxyl group at the C5 position; then, carrying out hydrolysis so as to remove a glycosyl group at the C13 position; then, carrying out a reaction so as to remove a hydroxyl group at the C13 position; and then, removing a hydroxyl protection group at the C5 position, carrying out hydroxyl oxidation so as to produce keto-carbonyl, and carrying out an oximation reaction, thereby finally obtaining a milbemycin oxime compound. According to the method, the milbemycin oxime compound product is synthesized from avermectins, which serve as the raw material, by a chemical synthesis method; and the chemical synthesis method has mature route and has no need of taking milbemycin, which is required to be obtained through fermentation, as the raw material, so that the cost and scarcity of the raw material are reduced, the large-scale industrial production is better facilitated, milbemycin oxime products are enriched, and thus, the shortage of domestic pet parasite treatment drugs is made up.
Owner:RINGPU TIANJIN BIOLOGICAL PHARMA

A kind of preparation method of milbexime

The invention provides a preparation method of milbemycin oxime. The preparation method of milbemycin oxime comprises the following steps: extracting milbemycins, namely taking milbe mycelia obtained through fermentation as a raw material, extracting, concentrating, extracting, concentrating, extracting again and concentrating again, so as to obtain milbemycin; preparing milbemycin ketone, namely taking the milbemycin as a raw material, establishing an oxidation reaction system, after reaction is finished, filtering, concentrating, extracting and concentrating the reaction system, so as to obtain an intermediate product milbemycin ketone; synthesizing milbemycin oxime, namely dissolving milbemycin ketone with methanol and dioxane, dropwise adding hydroxylamine hydrochloride solution and reacting, concentrating the reaction system, extracting, drying and concentrating, so as to obtain a milbemycin oxime crude product; and purifying milbemycin oxime, namely crystallizing the milbemycin oxime crude product with a mixed solvent of trichloromethane and normal heptane, dissolving a crystalline product with ethanol, dropwise adding into water while stirring for carrying out crystallization, filtering, and drying, so that the milbemycin oxime finished product is obtained. The preparation method of the milbemycin oxime has the advantages that productivity is greatly improved, and cost is obviously reduced, so that the preparation method provided by the invention is obviously better than an existing preparation method.
Owner:HUBEI HONCH PHARMA

A kind of separation and purification method of milbexime

The invention provides a milbemycin oxime separation and purification method. The milbemycin oxime separation and purification method comprises the following steps: carrying out crude separation on a sample to be separated through silicagel column chromatography, wherein the silicagel column chromatography adopts wet-process column packing and dry-method sample loading; purifying the product after crude separation by high performance liquid chromatography; concentrating the purified sample by a nanofiltration membrane to obtain concentrated solution; performing reduced-pressure vaporization to the concentrated solution, filtering and drying to obtain a refined product; dissolving the refined product, heating up, dropwise adding isooctyl alcohol, normal heptane or petroleum ether, cooling to be crystallized, and filtering to obtain a crystallized product; dissolving the crystallized product, filtering, dropwise adding filtrate into purified water stirred constantly, after dropwise adding, carrying out suction filtration to obtain a crystal transformation product; drying the crystal transformation product, crushing, and drying again to obtain milbemycin oxime finished product. The method can realize industrial production of milbemycin oxime in China firstly, and the product has high purity and high yield which are higher than those of the similar products at home and abroad.
Owner:HUBEI HONCH PHARMA

Method for simultaneously detecting contents of praziquantel, clofenoxine and milbemycin oxime

The invention provides a method for simultaneously detecting contents of praziquantel, clofenoxine and milbemycin oxime, which comprises the following steps: firstly preparing a blank solution, then preparing a test solution containing praziquantel, clofenoxine and milbemycin oxime, finally preparing a system applicability test solution, respectively injecting into a liquid chromatograph, and recording chromatograms under ultraviolet wavelength; according to the method, acetonitrile is taken as a diluent, octadecylsilane chemically bonded silica is taken as a chromatographic column in a liquid chromatograph, gradient elution is carried out, the flow rate is 1.0 mL/min, the column temperature is 30 DEG C, the ultraviolet detection wavelength is 230 nm, and the sample size is 20 mu L; the mobile phase A is a phosphoric acid solution with the content of 10-30 wt%, and the mobile phase B is acetonitrile with the content of 70-90 wt%; the detection method provided by the invention is simple and convenient, saves time, reduces cost, has good specificity, accuracy, linearity, durability and precision, and is suitable and accurate for detecting the content of the three components, so that the detection method can be used for daily detection of the pet medicine.
Owner:上海汉维生物医药科技有限公司
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products