The invention discloses fertilizer for preventing tobacco bacterial wilt and increasing the yield. The fertilizer comprises the following raw materials: cow dung, sheep dung, fish maw and gut, brewers grain, omphisa fuscidentalis hampson powder, arborvitate tops, katsuratree barks, tea straws, urea, superphosphate, monoammonium phosphate, potassium nitrate, potassium sulphate, potassium chloride, borax, zinc amino acid chelate, ferrous amino acid chelate, copper amino acid chelate, manganese amino acid chelate, molybdenum amino acid chelate, sodium metasillcate, chromium yeast, nanometer cobalt, gama-polyglutamic acid, polyaspartic acid, phosphorous acid, 3,4-dimethylpyrazole phosphate, a compound water-preservation agent and a microbial agent. The fertilizer organically combines four major functions of tobacco fertilization balancing nutrition, element cooperatively reinforcing function, biological antagonism restraining germs and soil conditioning preventing and treating source, greatly improves the prevention effect on tobacco bacterial wilt, and meanwhile has the effects of water retention and yield increasing.