The invention discloses a multi-wavelength laser, a preparation method and the application thereof. The multi-wavelength laser is at least composed of an encapsulation substrate, a thermoelectric cooler, a base plate, a piece of slide glass and a laser chip, wherein, the laser chip at least consists of a semiconductor substrate, distributed feedback Bragg raster display, a lower cladding, a lower barrier layer, an active region, an upper barrier layer, an upper cladding, the distributed feedback Bragg raster display and a metal electrode in sequence; the active region is internally provided with an active gain medium which is formed by a plurality of preconcerted quantum dot belts which are made of semiconductor dielectric material and have modulatory components and sizes, and each of the quantum dot belts internally contains a plurality of quantum dots having the same size and uniform distribution; the quantum dots are arranged in line, each of the quantum dot belts is corresponding to different lasing wavelengths, and the sizes of the quantum dots in the quantum dot belts are not the same as each other; the invention is characterized by good wavelength selectivity, high conversion quantum efficiency, high light output power, etc.