The invention relates to the technical field of composite membranes, in particular to an antibacterial composite membrane containing PEC, KGM and nano TiO2 and a preparation method thereof.The antibacterial composite membrane containing PEC, KGM and nano TiO2 is prepared from, by weight, 0.6%-1% of pectin (PEC), 0.5%-1.0% of konjac glucomannan (KGM), 0.5%-0.7% of glycerin, 0.5%-4.5% of nano TiO2 and the balance distilled water; the antibacterial composite film disclosed by the invention has excellent tensile property, high water vapor permeability and low oil permeability coefficient, and can promote the diffusion of moisture in fresh meat, reduce the moisture content of the meat and delay the decay of the meat; the loss of flavor substances can be reduced, the loss of fat can be blocked, and the flavor of fresh meat is kept; after the nano TiO2 is added into the antibacterial composite film, the mechanical property, the antibacterial activity, the barrier property and the like of the antibacterial composite film are obviously improved; the nano TiO2 can inhibit the breeding of microorganisms on the surface of food, can effectively make up the performance defects of the PEC/KGM composite film, and can enhance the antibacterial effect and mechanical properties of the PEC/KGM composite film.