The invention provides a refining production method of crude glycerin. Byproducts of grease ester exchange or hydrolyzation are used as ingredients, and byproducts of biodiesel can also be used as ingredients. The method comprises the following steps: acidizing treatment, decolourization, alkali return for neutralization, concentration for salt removal, ion exchange and concentration treatment, wherein the ion exchange step comprises the substep of sequentially using cations, anions, cations, anions and zwitterions to exchange resin posts in a mixed way, the operation temperature of the resin posts is controlled between 40 and 60 DEG C, the flow rate is 15 mL / min, and finally, the pure glycerin solution is collected. The process of the invention has the advantages of simple operation condition, low temperature, little pollution and high yield, and can avoid the deterioration of the glycerin at the high temperature, the yield is higher than 97 percent, and the product purity is higher than 99.2 percent. The invention also provides a multiplex mixed type anion-cation exchange resin post combination device applicable to the method.