The invention discloses a traditional Chinese medicine composition having efficacy of supplementing qi, nourishing yin and resisting weariness and a preparation method of the traditional Chinese medicine composition. The traditional Chinese medicine composition is mainly prepared from the following components of Chinese yams, haws, purslane herbs, papayas, hemp seeds, rhizoma polygonati odorati, licorice roots, ginkgo seeds, longans, lily bulbs, fructus phyllanthi, citrus medica, hippophae rhamnoides fruits, gordon euryale seeds, Chinese jujubes, fructus momordicae, honeysuckle flowers, Chinese wolfberry fruits and the like, and has the effects of supplementing qi, nourishing yin, clearing heat, promoting blood circulation, tonifying the liver and kidney, invigorating spleen to promote digestion, benefiting qi, producing the body fluid, reducing blood lipid and blood pressure, improving immunity, resisting weariness and the like, and is mainly suitable for symptoms of deficiency of both qi and yin, body fluid deficiency, thirst, weak health, weakness, poor appetite, high blood lipid, disorder glycometabolism and the like caused by work overtime, staying up late or all night, improper diet, irregular daily life and the like. Animal test proves that the traditional Chinese medicine composition has obvious efficacy of supplementing qi and resisting weariness, and is favorable forregulation of somatic function and enabling people to be away from sub health after being drunk for a long term.