The invention discloses a preparation method of a milk bottle, fruit, and vegetable cleaning agent. The milk bottle, fruit, and vegetable cleaning agent comprises following raw materials: honeysuckleflower, tea tree essential oil, coconut shell extract product, haw fruit, cordate houttuynia, and a surfactant. According to the preparation method, honeysuckle flower is taken as a main raw material,haw fruit and cordate houttuynia are adopted, and the surfactant, tea tree essential oil, coconut shell extract product, vitamin, and sodium bicarbonate are combined to prepare the milk bottle, fruit, and vegetable cleaning agent. Compared with commercially available cleaning agents, the advantages are that: using of a large amount of chemical preparations is avoided, and natural plant essence isadded; honeysuckle flower possesses wide spectrum antibacterial effect, and possesses inhibition effect on staphylococcus aureus, staphylococcus albus, typhoid bacillus, Shigella Castellani, diplococcus pneumoniae, and the like, and haw fruit and cordate houttuynia are adopted as auxiliary components, so that the milk bottle, fruit, and vegetable cleaning agent is capable of inhibiting bacteria on milk bottles, fruits, and vegetables, and protecting infant and adult health, and it is convenient for development and popularization.