The invention discloses a method for improving the anaerobic digestion gas-production performance of maize straws through directional acidification pretreatment, and belongs to the field of efficient resource utilization of maize straws. The method comprises the following steps: carrying out pretreatment on maize straws by using sodium hydroxide; acidifying the maize straws, and adding activated sludge into the pretreated maize straws, wherein relatively, 10-50 g of TS activated sludge is added into 52 g of TS maize straws; adding tap water until the tap water accounts for 80% of the total volume of a reaction container, mixing the obtained object, filling nitrogen into the reaction container, and hermetically fixing the container in a shaking table, wherein the temperature of the shaking table is kept at 40+/-2 DEG C; measuring the pH value by using a pH meter every time, and when the pH value is less than 5.5, stopping acidifying; and after methanation startup is completed, by taking an acidified discharged material as a raw material, carrying out feeding and discharging once a day, wherein a fed material and the discharged material are same in volume, and the HRT (hydraulic retention time) is 40 days. According to the invention, the separation of an acidification stage and a methanation stage in biological sciences is realized, and the effects of acidification bacteria groups and methane-producing bacteria groups in respective stage are enhanced, thereby facilitating the stable and efficient operation of an acidification phase and a methane-producing phase, and greatly improving the biodegradability and gas production performance of maize straws.