The invention discloses an organic fertilizer stack type fermentation method taking mushroom dregs as a substrate. The method comprises the following steps: s1, preparing a fermentation microbial inoculum; s2, treating a fermentation substrate; s3, mixing the fermentation raw materials; s4, fermenting the raw materials: fermenting the fermentation raw materials, specifically, conveying the fermentation raw materials into a windrow type fermentation tank through conveying equipment to form compost, monitoring the compost temperature in the fermentation period, and turning the compost pile oncewhen the fermentation temperature is higher than 65 DEG C; when the fermentation temperature is always lower than 65 DEG C, turning the compost pile once every 5-6 days; when the fermentation temperature is reduced to 30 DEG C, ending the fermentation and preparing an organic fertilizer; s5, subsequent processing; s6, drying; and s7, packaging. According to the present invention, the raw materialscan be crushed and effectively mixed so as to uniformly distribute the fermentation substrate and the fermentation microbial inoculum, such that the fermentation effect is significantly improved, thewaste of the bacterial residue can be avoided, the nutrients in the bacterial residue can be effectively utilized, the quality of the organic fertilizer is significantly improved, the operation is simple, and the method is suitable for large-scale processing.