The invention relates to a room-temperature curing polyurethane material as well as a preparation method and application thereof, belongs to the technical field of high polymer materials and solves the problem of how to provide a polyurethane material which is green and environmentally friendly, capable of being cured at a room temperature, good in fluidity, long in service life, high in strength,resistant to humidity and heat, simple in operation process, adjustable and controllable in curing time and strong in bonding performance with metals. The preparation method provided by the inventioncomprises the following steps: firstly mixing pre-dehydrated polyol with isocyanate and reacting to obtain a NCO-terminated polyurethane pre-polymer, namely a component A; then blending polyol and asmall-molecule chain extender, dehydrating, adding a catalyst and uniformly stirring to obtain a component B; finally weighing the component A and the component B according to a chain expansion coefficient of 0.95, sealing and sub-packaging to obtain the polyurethane material. The material is green and environmentally friendly, long in storage life, easy to uniformly mix, good in fluidity, good bonding performance with the metals, capable of being cured at a room temperature, flexible, adjustable and controllable in curing time, high in strength after curing, good in humidity and heat resistance and suitable for metal pipe pouring molding.