The invention provides fat beef essence. The fat beef essence consists of methyl (2-methyl-3-furyl) disulfide, bis(2-methyl-3-furyl) disulfide, 2-methyl-3-furanyl mercaptan, 4-methyl-4-mercapto-2-
pentanone, 12-methyltridecanal, 2-methyl-4=
tetrahydrofuran-3-
thiol liquid, trans-trans-2,4-decadienal, octanoic acid, 4-methyl octanoic acid, 2-
acetylpyrazine, 2,3,5-trimethylpyrazine, furanone, 2,4,6-trisisobutyl-1, 3,5-dithiazine, 2-acetylthiazole, dodecalactone, 2, 3-butanedione, 3-hydroxy-2-
butanone, hot 
ginger oil and salad oil. The invention further provides a preparation method of the fat beef essence. The fat beef essence is prominent in 
flavor, natural and harmonious in fragrance, mellow, vivid and layered in 
aroma and lasting in fragrance, and can be used for flavoring a hot pot and a food 
seasoning.