The invention discloses a preparation method of low-molecular-weight
takifugu rubripes fishbone
collagen peptide. The preparation method comprises the following steps: under a low-temperature condition, naturally unfreezing
takifugu rubripes fish bones, washing the fish bones with
distilled water,
cutting the fish bones into pieces, adding a NaOH solution and stirring to remove impure
protein, adding an EDTA-2Na solution and stirring to remove mineral substances, adding an isopropanol
aqueous solution and stirring to remove fat, then washing the fish bones with
distilled water,
drying the fish bones in a
drying oven, and crushing the fish bones with a universal
crusher to obtain the
takifugu rubripes fish bones. Adding water and compound
protease, performing enzymolysis in a water bath, inactivating in a boiling water bath, centrifuging, taking supernatant, and performing
freeze drying to obtain
collagen peptide powder. The preparation raw materials are low in cost and easy to obtain, and the content of crude
protein and collagen is high. According to the extraction method, a relatively mild
enzyme method is selected for preparation, the influence on the
collagen peptide is relatively small,
alkaline protease and
arenga pinnata
plant protease are combined for use, Neutrase
enzyme and Alcalase
enzyme are combined for use, and a synergistic effect is achieved.