The application discloses a double-temperature-zone PCR micro-fluidic
chip driven by biaxial
centrifugal force for space application, which can provide simulated gravity under microgravity conditions through
centrifugal force, and can eliminate the problem of air bubbles generated in the qPCR process; meanwhile, a variable-temperature mode of fixed-temperature-zone driving liquid is adopted, so that the
power consumption demand in the amplification process is reduced. X and Y axes which are perpendicular to each other are divided along the center of the
chip, and four regions are formed; each region contains a pipeline
system with the same structure. Each pipeline
system is as follows: an amplification
system buffer channel is connected to an amplification system quantitative
pool through a first capillary valve; a side of the amplification system quantitative
pool is provided with a channel connected to a waste liquid
pool, and liquid more than the capacity of the amplification system flows into the waste liquid pool; the other side of the amplification system quantitative pool is connected to a
dry powder preset channel through a second capillary valve; the
dry powder preset channel is connected to an amplification pool; the direction extension line of the amplification pool and the x axis and the origin form an
acute angle; the amplification pool is divided into low-temperature and high-temperature constant-temperature zones. A paraffin oil sampling port is located in the middle of the amplification pool.