The invention relates to the technical field of biological experiment equipment, in particular to a
living cell vacuum
irradiation device and an experiment method. The
living cell vacuum
irradiation device comprises a driving mechanism which is provided with a first
flange and a telescopic rod; the
vacuum chamber is provided with a first port and a second port; the
living cell carrier is used for keeping
cell activity; wherein the first
flange is connected with the first port, the first end of the telescopic rod extends into the
vacuum chamber from the first port, and the living
cell carrier is mounted at the first end of the telescopic rod; the second port is connected with a
proton accelerator, and the living
cell carrier is driven by the telescopic rod to adjust the position relative to the second port so as to receive
ion irradiation. In the invention, by optimizing the structure of the irradiation device, the living
cell carrier can be placed in the
vacuum chamber, so that the
ion beam directly irradiates the living cells, and the irradiation of the living cells can be implemented by using a low-energy accelerator of which the
proton energy is more than 0.32 MeV.