The invention relates to a method for preparing mesoporous nano particles with special appearance based on an anion surfactant, and belongs to a method for preparing mesoporous nano particles. The method comprises the following steps of: dissolving 1.0 millimole of Sar-Na in 30 mL of deionized water at the temperature of between 14 and 18 DEG C, dripping 2.0 mL of 0.1 M hydrochloric acid, regulating the pH value to 6.0 to 6.8 slowly, stirring for 30 minutes, dripping 1.0 mL of tetraethyl orthosilicate (TEOS) and 0.1 mL of 3-aminopropyl trimethoxysilane (APMS), stirring a mixture at room temperature for 10 minutes, putting into a kettle, standing at the temperature of 15 DEG C for 2 hours, transferring the reaction kettle into a drying oven of 80 DEG C, and crystallizing for 20 hours; cooling to the room temperature, opening the kettle, centrifuging and washing; drying at the temperature of 40 DEG C for 48 hours; and performing reaction to generate two products, wherein one product is positioned on a solution and is named AMS-U, the other product is dispersed in a substratum solution and is named AMS-R, superstratum substances are mesoporous hollow spheres, and substratum products are solid spheres with mesoporous structures. The method has the advantages that two mesoporous nano particles are prepared in one step and are distributed in two layers, the superstratum substances are the mesoporous hollow spheres, the substratum substances are the solid spheres, and the hollow spheres and the solid spheres can be separated by a physical method.