The invention discloses a vulcanization molded single-component high-thermal-conductivity interstitial interface material with moisture and a preparation method of the interface material, relates to the technical field of materials, and in particular relates to the high-thermal-conductivity interface material. The vulcanization molded single-component high-thermal-conductivity interstitial interface material with moisture is mainly prepared from the following components in parts by weight: 100 parts of alkoxy silicone oil, 100-1500 parts of thermal conductive powder, 0-10 parts of a chain extender, and 1-10 parts of a catalyst. The interface material provided by the invention takes the alkoxy silicone oil as a matrix, and the thermal conductive powder and the chain extender are added, so that the product has the advantages of good thermal conductivity, curing performance, low stress, good pressure absorption performance, a thin adhesive layer, high adhesion performance, convenient production, low costs, convenient sizing, on-line shaping and the like.