The invention discloses a natural dyestuff dyeing and finishing process of polyester fabric. The natural dyestuff dyeing and finishing process of the polyester fabric comprises pretreatment, preshaping, modification treatment, dyeing, mordant removing, soaping, fixation, finishing, drying, shaping, calendering and winding. According to the natural dyestuff dyeing and finishing process of the polyester fabric, helicase and alhpa-amylase are adopted to conduct pretreatment, modification treatment is radically conducted on the performance of the polyester fabric through a acetic acid aqueous solution of chitosan, hydrogen peroxide and epoxy chloropropane, and the dyeing rate of the fabric is increased; in the dyeing process, a large amount of alkaline matter and inorganic salt are not used, a levelling agent and diffusant are added, the level-dyeing property is improved, natural dyestuff is used, therefore, environmental pollution is avoided, and requirements of green environmental protection are met; modification treatment and fire-retardant treatment are conducted on the fabric through montmorillonite, and the fire resistance of the fabric is high; anti-yellowing reagents are used in the preshaping and the shaping, and the final fabric is not prone to yellowing.