The invention provides a mineralized plant nutrient solution as well as a preparation method and application thereof, and belongs to the technical field of microelement fertilizer extraction. The micalex and the vermiculite are used as mineral raw materials, the micalex is rich in phosphorus, potassium, silicon, calcium, magnesium and iron elements and trace chlorine, manganese, molybdenum and zinc elements, and the vermiculite is rich in silicon, magnesium and iron elements; a sulfuric acid solution is used as an extracting solution of trace elements, on one hand, sulfuric acid can react with various metal ions in raw materials, the trace elements are extracted from micalex and vermiculite in the form of sulfate, and a stable sulfate solution is formed after the trace elements are passivated by sulfolobus acidocaldarius; when being used for irrigating saline-alkali soil plants, the plant photosynthesis can be enhanced, the nutrient absorption and utilization rate of crops is increased, the disease resistance and average quality of the plants are enhanced, and the yield of the plants is further increased; on the other hand, the residual free sulfuric acid can be used for transforming the saline-alkali soil, so that the salinity in the saline-alkali soil is reduced, and the pH value of the soil tends to be neutral.