The invention discloses a method for testing the impedance of an RFID tag antenna, and aims to provide a method for rapidly, simply and accurately testing the impedance of the RFID tag antenna. According to the technical scheme, the method comprises the steps that firstly, a vector network analyzer is calibrated, a test fixture is connected to the vector network analyzer, a dual-port differentialtest model is established to replace a single-port test model of a traditional antenna impedance test, and an extension structure is connected with the vector network analyzer through a coaxial line;the two modes of open-circuit calibration and short-circuit calibration are measured, and an average value of the two modes is selected; after a series of calibration and port migration are completed,the to-be-tested tag antenna is welded with the test fixture, required related test S parameters are obtained from the vector network analyzer, data are exported to simulation calculation mathematical software Matlab for data processing, and tested antenna impedance is obtained.