The invention discloses a preparation method for a silver-loaded nano particle temperature stimuli responsiveness hybrid nanofiber membrane. The method comprises the steps that temperature stimuli responsiveness monomers and functional monomers with heat reactivity groups are copolymerized to form a polymer with the temperature stimuli responsiveness by adopting a free radical polymerization method; the polymer is dissolved in deionized water at room temperature, proper silver nitrate is added into the deionized water with the polymer, the mixture is mixed evenly, and the silver ions in the mixture are reduced to silver nanoparticles through ultraviolet light irradiation; electrostatic spinning is conducted on a polymer aqueous solution with the silver nanoparticles, and the silver-loaded nano particle temperature stimuli responsiveness hybrid nanofiber membrane is obtained. According to the preparation method for the silver-loaded nano particle temperature stimuli responsiveness hybrid nanofiber membrane, the hybrid nanofiber membrane is prepared by adopting an electrostatic spinning method, in the preparation process, water is adopted to serve as the solution, mild conditions are achieved, and harmful and poisonous products are not produced; the silver-loaded nano particle temperature stimuli responsiveness hybrid nanofiber membrane can be stored in the water stably, dissociation does not occur, the temperature stimuli responsiveness is achieved, and the silver-loaded nano particle temperature stimuli responsiveness hybrid nanofiber membrane can be used for catalytic reaction or a substrate with the surface-enhanced Raman spectrum or antiseptic dressing.