44 results about "Stropharia coronilla" patented technology

The invention belongs to the technical field of edible fungus cultivation, and relates to a preparation method of a S. rugosoannulata liquid reduction strain, which comprises the following steps: (1) activation of a S. rugosoannulata strain: inoculating S. rugosoannulata mycelium to a PDA culture medium, and culturing at 20-28 DEG C for 10-15 days to obtain a mother strain; (2) preparing a first-stage liquid strain: transferring the mother strain into a first-stage liquid strain culture medium, and culturing in a shaker in a dark place at the speed of 150-180 rpm and the temperature of 20-28 DEG C for 7-10 days to obtain the first-stage liquid strain; (3) preparing a secondary liquid strain: inoculating the primary liquid strain into a secondary liquid strain culture medium, and fermenting at 20-28 DEG C for 3-5 days to obtain the secondary liquid strain; and (4) preparing a liquid reducing strain: mixing the mycelium pellets of the secondary liquid strain with a reducing strain culture solution to obtain the liquid reducing strain. The invention also relates to a method for cultivating stropharia rugoso-annulata sporocarp by using the liquid reduction strain. According to the method, the culture period of the stropharia rugoso-annulata strain can be greatly shortened.

The invention discloses a stropharia rugoso-annulata strain breeding method. The breeding method comprises the following steps of preparing a stropharia rugoso-annulata rejuvenation culture medium, inoculating stropharia rugoso-annulata mother strains to the rejuvenation culture medium for variable-temperature rejuvenation pure culture, and cultivating stress-resistant and high-activity strophariarugoso-annulata mother strains; preparing a shake-flask liquid culture medium, inoculating the rejuvenated stropharia rugoso-annulata mother strains onto the shake-flask liquid culture medium, and cultivating the stropharia rugoso-annulata shake-flask liquid strain by adopting a shake-flask liquid submerged fermentation process; preparing a fermentation tank fermentation culture medium, inoculating the cultured shake-flask liquid strain onto the fermentation tank fermentation culture medium, and cultivating a liquid submerged fermentation strain of stropharia rugoso-annulata by adopting a fermentation tank liquid submerged fermentation process. A composting fermentation method is adopted, the stropharia rugoso-annulata cultivar culture medium is prepared, the prepared cultivar fermentation culture medium is put into a cultivar breeding tray hole, then a cultivated stropharia rugoso-annulata liquid submerged fermentation strain is inoculated to the cultivar fermentation culture mediumin the breeding tray hole through an inoculation gun, and the stropharia rugoso-annulata cultivar is obtained through an open solid fermentation process.

The invention provides an organic cultivation method for stropharia rugoso-annulata employing wild miscanthus as a main ingredient. The method comprises the steps of field selecting, selecting and treatment of a cultivation material, land preparation and compartment making, sowing, spawn running management, mushroom emergence stage management and harvesting, wherein the wild miscanthus serves as the cultivation material, and the stropharia rugoso-annulata can normally grow without adding other adjuvants. According to the method provided by the invention, a renewable wildlife resource, i.e., the miscanthus, which cannot be completely developed at present, can be fully utilized, and sources of cultivation raw materials are extremely enriched; and meanwhile, the stropharia rugoso-annulata iscultivated by using the wild miscanthus resources, so that pollution caused by pesticides and heavy metals can be avoided from source, the product quality is improved, and the benefit of cultivation is improved.

The invention discloses a greenhouse and indoor cultivation method for stropharia rugoso-annulata. The greenhouse and indoor cultivation method for the stropharia rugoso-annulata comprises the following steps: selecting a planting environment; ridging and ridge bottom strengthening; mixing soil with materials; performing stacking and spawn running; performing stirring in a spacing manner; inoculating auxiliary materials; performing spawn running and fruiting management; and picking the stropharia rugoso-annulata on schedule. The production method disclosed by the invention has the beneficial effects that the situation that the stropharia rugoso-annulata strain is soaked to be damaged due to more accumulated water at the ridge bottom is avoided, the stropharia rugoso-annulata is watered more uniformly, and the situation that the production is non-uniform due to more watering and less watering at some places is avoided, material piles in the step IV are turned over, shaken and scattered to the site on the other side from top to bottom by using a steel fork tool, the material piles are stacked layer by layer, watering is performed while shaking and scattering are performed according to dryness and wetness, the material piles are turned over every 2-3 days, turning over and stirring are performed for 2-3 times, the soil material can be evenly and fully fermented up and down, besides, shelf type cultivation is adopted indoors, space is fully utilized, and cultivation amount can be increased.

The invention relates to a stropharia rugoso-annulata comprehensive culture medium. The culture medium comprises a basic culture solution, the basic culture solution is prepared by decocting 1.5% by mass of stropharia rugoso-annulata fresh sporocarp with tap water meeting the drinking standard of domestic water, and 1.0-2.0% by mass of cane sugar, 0.10-0.50% by mass of protein powder and 0.20-0.30% by mass of yeast powder are added into the basic culture solution. The technical modes that a traditional culture medium is single in nutrition and a conventional method is long in strain preparation period are broken through, the defects that the fruiting period is short and the yield is low are overcome, the liquid strain is adopted for preparing and cultivating the strain, and the comprehensive culture medium is used for cultivating the stropharia rugoso-annulata. Practice shows that in the cool temperate zone, compared with the conventional method for cultivating the stropharia rugoso-annulata, the method for cultivating the stropharia rugoso-annulata has the advantages that under the natural climatic condition, artificial cultivation can be implemented 15 days earlier, the fruiting period is prolonged by about 20 days, and the biological conversion rate can be increased by 15%-20%.

The invention discloses application of stropharia rugosoannulata polysaccharide, a resistance inducer and a grape cultivation method. Research finds that when the stropharia rugosoannulata polysaccharide is prepared into a resistance inducer for grape cultivation, the disease resistance of grape plants can be improved, pesticide use is reduced, the prevention and treatment effect on grape downy mildew and grape anthracnose diseases is good, the yield and quality of grape fruits can be improved, and the stropharia rugosoannulata polysaccharide is safe to grapes under the treatment concentration. The stropharia rugosoannulata polysaccharide can be independently prepared into a resistance inducer and can also be compounded with other components such as amino-oligosaccharin, calcium amino acidchelate and magnesium amino acid chelate, and after compounding, the prevention and treatment effect of the inducer can be further improved.

The invention discloses a stropharia rugoso-annulata culture medium and a preparation method and application thereof.According to the stropharia rugoso-annulata culture medium, perishable organic waste is adopted as raw materials and comprises garden waste and pre-meal waste, and the garden waste comprises but not limited to branches and fallen leaves; the pre-meal garbage comprises but is not limited to fruits, vegetables, food leftovers and melon and fruit peels discarded in family daily life. According to the method, the perishable organic waste is used for manufacturing the culture medium to replace a traditional stropharia rugoso-annulata culture medium raw material, the idea of resource cyclic utilization is exerted and practiced to the maximum extent, the resource utilization level of the perishable organic waste is greatly improved, and the new mode that non-woven bags are used for indoor culture is achieved; therefore, a traditional stropharia rugoso-annulata cultivation mode is broken, the cultivation technology is simplified, and the cultivation process is optimized and simplified.

The invention discloses a stropharia rugosoannulata liquid strain incubator. The stropharia rugosoannulata liquid strain incubator is characterized by comprising a liquid stock culture tank (1), a temperature control magnetic turntable (2) and a combined stirrer (3), wherein the liquid stock culture tank (1) is arranged above the temperature control magnetic turntable (2), stock culture liquid containing liquid mother strains is loaded in the liquid stock culture tank (1), and the combined stirrer (3) is placed in the stock culture liquid; and the temperature control magnetic turntable (2) rotates to drive the combined stirrer (3) to rotate, and non-contact stirring and heating of the stock culture liquid are achieved. When the incubator is used for strain culture, compared with solid stock culture, the stock culture time can be shortened by 40-60 days, and the spawn running time of cultivated species can be shortened by 30-50 days.