A medical stone toughened polyvinyl alcohol film for preservation of fruits and vegetables is characterized by being prepared from raw materials as follows: camellia seeds, mint leaves, dried ginger, folium artemisiae argyi, clove flowers, acetone, anhydrous sodium sulfate, medical stone, whey protein, propylene glycol, agaric polysaccharide, corn starch, caustic soda, vinyl acetate, polyvinyl alcohol, an ammonium persulfate solution, hydroxyethyl acrylate, glyceryl monooleate, glycerin and a proper amount of deionized water. The medical stone toughened polyvinyl alcohol film for preservation of fruits and vegetables has good mechanical performance, is stable in structure and good in air permeability, also has a good antibacterial function, can reduce endogenous metabolism ageing of the fruits and vegetables and rotting caused by exogenous microorganisms, substantially prolongs the preservation time and effectively keeps the quality of the fruits and vegetables.