The invention relates to a fiber and filler mixed enzymatic defibrination method which comprises the steps of high concentration and low concentration series beating, paste and filler premixed beating, and paste and beating enzyme premixed beating. Firstly, pre-treating fibers by beating enzyme, so as to enable the fibers to be soft and convenient to beat; secondly, adding fillers when concentrating the paste into a higher concentration through a press master; and conducting beating through a high consistency refiner. According to the method provided by the invention, fiber intensity is improved through three modes, tensile strength, bursting strength, folding endurance and cohesion of paper can be efficiently improved; identical paper strength can be maintained under the condition of improving 2 percent to 3 percent of paper ash, furthermore, beating energy consumption can be greatly decreased under the action of beating enzyme. Through the application of the method in a paper mill, macrofiber usage can be decreased, paper ash part can be improved, cost of a ton of paper can be greatly decreased, and product competitiveness can be improved.