The invention relates to a preparation method of a self-adhesive and degradable disposable tablecloth. The self-adhesive and degradable disposable tablecloth comprises an adhesive layer, an inner layer and an outer layer and is composed of components including C6LLDPE (carbon 6 linear low-density polyethylene), mLLDPE (metallocene linear low-density polyethylene), PIB (polyisobutylene), starch, polylactic acid, glycerinum, bulking agent, soy protein, C8LLDPE, LDPE (low-density polyethylene), cycloolefin, anti-blocking agent and calcium carbonate. The self-adhesive and degradable disposable tablecloth prepared through the preparation method has the advantages of being non-toxic, environmentally friendly, pollution-free, light in weight, low in water absorption, high in breaking elongation,biologically degradable and free from recycling.